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| Description | |
|---|---|
| Catalogue Number | AP182P |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Donkey Anti-Rabbit IgG Antibody, HRP conjugate, Species Adsorbed |
| Background Information | Immunoglobulin g (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants. |
| Product Information | |
|---|---|
| Format | HRP |
| Presentation | Lyophilized. Buffer = 0.01 M Sodium Phosphate, 0.25 M NaCl, pH 7.6 with 15 mg/mL BSA. Contains no preservative. RECONSTITUTION: Reconstitute with 500 μL of sterile distilled water. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Donkey anti-Rabbit IgG Antibody, HRP conjugate, Species Adsorbed is an antibody against Donkey Rabbit IgG for use in ELISA & WB. |
| Key Applications |
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| Application Notes | EIA and Western blots: 1:5,000-1:100,000. Immunohistochemistry: 1:500-1:5,000. Western blotting with ECL substrates: 1:10,000-1:200,000. Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Donkey |
| Specificity | Rabbit IgG |
| Species Reactivity |
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| Antibody Type | Polyclonal Antibody |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain lyophilized product at 2-8°C for up to 12 months. After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles. WARNING: Use of sodium azide as a preservative will substantially inhibit the enzyme activity of HRP. |
| Packaging Information | |
|---|---|
| Material Size | 500 µL |