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| Description | |
|---|---|
| Catalogue Number | AP200B |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Goat Anti-Mouse light chain Antibody, biotin conjugate |
| Background Information | Antibody molecules typically comprise two immunoglobulin light chains covalently bound to a pair of heavy chains. Immunoglobulin light chains occur in two types, designated by the Greek letters kappa and lambda. Kappa and gamma light chains are approximately 250 amino acids in length with an average mass of about 25 kDa. The ratio of kappa to lambda found in the immunoglobulin population varies by species. |
| Product Information | |
|---|---|
| Format | Biotin |
| Presentation | Lyophilized powder from a solution with 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 0.05% Sodium Azide. 15 mg/mL BSA as stabilizer. Purified by immunoaffinity chromatography. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect Mouse light chain using this Goat anti-Mouse light chain Antibody, biotin conjugate validated for use in ELISA, FC, WB, IC, IH(P). |
| Key Applications |
|
| Application Notes | Suggested dilutions: Western Blotting: 1:20,000 - 1:400,000 of the 1 mg/mL stock ELISA: 1:20,000 - 1:400,000 of the 1 mg/mL stock Immunohistochemistry: 1:500 - 1:5,000 of the 1 mg/mL stock Immunocytochemistry: 1:500 - 1:5,000 of the 1 mg/mL stock Flow Cytometry: 1:200 - 1:1,000 of the 1 mg/mL stock Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | Prepared from purified mouse IgG light chain. |
| Epitope | Kappa light chain |
| Host | Goat |
| Specificity | The antibody reacts strongly with native primary antibodies primarily with kappa light chains. It is not suitable for detecting lambda light chains. The antibody does not react with the heavy chain of mouse IgG. The antibody has been tested by ELISA and adsorbed to ensure minimal cross-reaction with bovine, goat, horse, human, rabbit, rat, and sheep immunoglobulins. |
| Isotype | IgG |
| Species Reactivity |
|
| Species Reactivity Note | Minimal cross-reaction with bovine, goat, horse, human, rabbit, rat, and sheep. |
| Antibody Type | Polyclonal Antibody |
| Purification Method | ImmunoAffinity Purified |
| Molecular Weight | 25 kDa |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain lyophilized product at 2°-8°C for up to 12 months. After reconstitution the product is stable for six weeks at 2°-8°C in the dark as an undiluted liquid. Reconstitute vial with of distilled water (addition of 0.5 mL water will yield a final concentration of 1 mg/mL). For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze thaw cycles. Keep away from light. |
| Packaging Information | |
|---|---|
| Material Size | 0.5 mg |