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Millipore Detect Mouse Igg Using This Goat Anti-Mouse Igg Antibody, (H+L) Fitc Conjugate Validated For Use In If. - Mill (Additional S&H or Hazmat Fees May Apply)
The reagent is an affinity purified antibody from goat. The purified antibody is conjugated to fluorescein and stabilized in buffer.
Background Information
Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
Product Information
Format
FITC
Presentation
Lyophilized. Buffer = 0.02M Phosphate Buffer, 0.25 M NaCl, pH 7.6 with 15 mg/mL BSA and 0.1% Sodium azide. Reconstitute with 1 mL of sterile distilled water.
Quality Level
MQ100
Applications
Application
Detect Mouse IgG using this Goat anti-Mouse IgG Antibody, (H+L) FITC conjugate validated for use in IF.
Key Applications
Immunofluorescence
Application Notes
F/P RATIO: 4-6
Immunohistochemistry: 1:200-1:500 (1-4)
Immunocytochemistry: 1:200-1:500 (1-4)
Flow Cytometry: 1 μg per 1x 10E6 cells (1,2,4) FITC absorption emission is 488-492nm, its emission peak is 520nm.
Optimal working dilutions must be determined by the end user.
Biological Information
Concentration
1.15 mg/mL
Host
Goat
Specificity
Specific for mouse IgG, heavy and light chain. The cross-reactivities of anti-mouse IgG antibody are tested in an ELISA. Minimum cross-reactivity to human IgG.
Species Reactivity
Mouse
Antibody Type
Polyclonal Antibody
Purification Method
ImmunoAffinity Purified
Product Usage Statements
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Maintain lyophilized product at 2–8°C for up to 12 months after date of receipt. After reconstitution the product is stable for several weeks at 2–8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles. Protect from light.
