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| Description | |
|---|---|
| Catalogue Number | AP308P |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Goat Anti-Mouse IgG Antibody, (H+L) HRP conjugate |
| Overview | The reagent is an affinity purified antibody from goat. The purified antibody is conjugated to horseradish peroxidase (HRP) and stabilized in buffer. |
| Background Information | The reagent is an affinity purified antibody from goat. The purified antibody is conjugated to horseradish peroxidase (HRP) and stabilized in buffer. |
| Product Information | |
|---|---|
| Format | HRP |
| Presentation | Purified Goat IgG conjugated to horseradish peroxidase in buffer containing liquid in 0.01M PBS, pH7.1 with 15mg/mL BSA and 0.1% Kathon |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Goat anti-Mouse IgG Antibody, (H+L) HRP conjugate is validated for use in ELISA, IC, IH, WB for the detection of Mouse IgG. |
| Key Applications |
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| Application Notes | ELISA: 1:3,000 dilution can be used (Coligan, J., 1997; Harlow, E., 1988). Immunohistochemistry/immunocytochemistry: 1:200-1:500 dilution can be used (Bullock, g.R., 1982; Javois, L.C., 1994). Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Concentration | 1 mg/mL |
| Host | Goat |
| Specificity | Specific for mouse IgG, heavy and light chain. The cross-reactivities of anti-mouse IgG antibody are tested in an ELISA. Minimum cross-reactivity to human IgG. |
| Isotype | IgG |
| Species Reactivity |
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| Antibody Type | Polyclonal Antibody |
| Purification Method | Affinity Purfied |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Western Blot, chromogenic: 1:200-1:500 dilution can be used (Coligan, J., 1997; Harlow, E., 1988). Western Blot, chemiluminescent: 1:2,000-1:5,000 dilution can be used (Coligan, J., 1997). |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | The undiluted antibody is stable at 2-8°C for 12 months. Do not store in a diluted format. DO NOT FREEZE. |
| Packaging Information | |
|---|---|
| Material Size | 1 mL |