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Millipore Goat Anti-Mouse Igm Μ Chain Antibody, Alkaline Phosphatase Conjugate, Species Adsorbed Detects Level Of Mouse Igm Μ Chain & Has Been Published & Validated For Use In Elisa. - Mill (Additional S&H or Hazmat Fees May Apply)
IgM constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and predominates in certain antibody responses such as natural blood group antibodies. IgM (with IgD) is the major immunoglobulin expressed on the surface of B cells. The gene for the mu constant region contains four domains separated by short intervening sequences.
Product Information
Format
Alkaline Phosphatase
Presentation
Purified by affinity chromatography on mouse IgM covalently linked to agarose. Liquid in 50mM Tris/1mM MgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative.
Quality Level
MQ100
Applications
Application
Goat anti-Mouse IgM µ chain Antibody, Alkaline Phosphatase conjugate, Species Adsorbed detects level of Mouse IgM µ chain & has been published & validated for use in ELISA.
Key Applications
ELISA
Application Notes
ELISA: 1:2,000-1:4,000
Optimal working dilutions must be determined by the end user.
Biological Information
Immunogen
Prepared from purified mouse IgM µ chain. Pooled antisera from goats hyperimmunized with mouse IgM paraproteins.
Epitope
IgM µ chain
Host
Goat
Specificity
The antibody reacts with the heavy chain of mouse IgM as demonstrated by ELISA and flow cytometry. Minimal cross reactivity with human immunoglobulins.
Species Reactivity
Mouse
Species Reactivity Note
Reacts with heavy chain of mouse IgM. Absorbed for mouse IgG1, IgG2a, IgG2b, IgG3 and IgA, pooled human sera and purified human paraproteins. Minimal cross reactivity with human immunoglobulins. Reactivity with other species has not been determined.
Antibody Type
Polyclonal Antibody
Purification Method
Affinity Chromatography
Product Usage Statements
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Maintain refrigerated at 2°-8°C under sterile conditions for up to twelve months from date of receipt. For long term storage, aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
