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Millipore Goat Anti-Mouse Igm+Igg+Iga (H+L) Antibody, Alkaline Phosphatase, Species Adsorbed Detects Level Of Mouse Igm+Igg+Iga & Has Been Published & Validated For Use In ELISA. - Mill (Additional S&H or Hazmat Fees May Apply)
Goat Anti-Mouse IgM+IgG+IgA (H+L) Antibody, Alkaline Phosphatase, Species Adsorbed
Background Information
IgG is the most abundant immunoglobulin compose of two heavy chains and two light chains. Each molecule has two antigen binding sites. IgG has 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%). IgM constitutes about 10% of serum immunoglobulins. IgM (with IgD) is the major immunoglobulin expressed on the surface of B cells. Monomeric IgA constitutes 5-15 % of the serum immunoglobulins whereas dimeric IgA is localized to mucosa surfaces.
Product Information
Format
Alkaline Phosphatase
Presentation
Purified by affinity chromatography on pooled mouse IgM+IgG+IgA covalently linked to agarose. Liquid in 1.0 mL of stock solution in 50mM Tris/1mM MgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative.
Quality Level
MQ100
Applications
Application
Goat anti-Mouse IgM+IgG+IgA (H+L) Antibody, Alkaline Phosphatase, Species Adsorbed detects level of Mouse IgM+IgG+IgA & has been published & validated for use in ELISA.
Key Applications
ELISA
Application Notes
ELISA: 1:2,000-1:4,000
Optimal working dilutions must be determined by the end user.
Biological Information
Immunogen
Prepared from pooled antisera from goats hyperimmunized with mouse IgM, IgG and IgA paraproteins.
Epitope
Mouse IgM, IgG, IgA
Host
Goat
Specificity
The antibody reacts with the heavy and light chains of mouse IgM, IgG1, IgG2a, IgG2b, IgG3 and IgA as demonstrated by ELISA and flow cytometry. Minimal cross reactivity with human immunoglobulins.
Species Reactivity
Mouse
Species Reactivity Note
Reacts with heavy and light chains of mouse IgM, IgG1, IgG2a, IgG2b, IgG3 and IgA. Absorbed for human sera and purified human paraproteins. Minimal cross reactivity with human immunoglobulins.
Antibody Type
Polyclonal Antibody
Purification Method
Affinity Chromatography
Product Usage Statements
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Maintain refrigerated at 2°-8°C under sterile conditions for up to twelve months from date of receipt. Store long term at -20°C.
