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Millipore Goat Anti-Mouse Igg Γ Chain Antibody, Alkaline Phosphatase Conjugate, Species Adsorbed Is An Antibody Against Mouse Igg Γ Chain For Use In ELISA. - Mill (Additional S&H or Hazmat Fees May Apply)
IgG is one of the most abundant protein in human serum. IgG is important for our defence against microorganisms and the molecules are produced by B-lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination.
Product Information
Format
Alkaline Phosphatase
Presentation
Purified by affinity chromatography on pooled mouse IgG covalently linked to agarose. Liquid in 50mM Tris/1mM MgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative.
Quality Level
MQ100
Applications
Application
Goat anti-Mouse IgG γ chain Antibody, Alkaline Phosphatase conjugate, Species Adsorbed is an antibody against Mouse IgG γ chain for use in ELISA.
Key Applications
ELISA
Application Notes
ELISA: 1:2,000-1:4,000
Optimal working dilutions must be determined by the end user.
Biological Information
Immunogen
Prepared from purified mouse IgG γ chain. Pooled antisera from goats hyperimmunized with mouse IgG paraproteins.
Epitope
IgG γ chain
Host
Goat
Specificity
The antibody reacts with the heavy chain of mouse IgG1, IgG2a, IgG2b and IgG3 as demonstrated by ELISA and flow cytometry. Minimal cross reactivity with human immunoglobulins.
Species Reactivity
Mouse
Species Reactivity Note
Reacts with heavy chain of mouse IgG1, IgG2a, IgG2b and IgG3. Absorbed for mouse IgM and IgA, pooled human sera and purified human paraproteins. Minimal cross reactivity with human immunoglobulins.
Antibody Type
Polyclonal Antibody
Purification Method
Affinity Chromatography
Product Usage Statements
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Maintain refrigerated at 2°-8°C under sterile conditions for up to twelve months from date of receipt. For long term storage, aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
