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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Am, B, Ca, Ch, H, Mk | ELISA, IF, IH(P), WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | CBL202 |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Anti-Vimentin Antibody, clone VIM 3B4 |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Protein A Purified immunoglobulin presented as lyophilized material. Reconstitute with 1ml distilled water. Final solution is PBS, pH 7.4, containing 0.5% BSA and 0.09% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Vimentin Antibody, clone VIM 3B4 is validated for use in ELISA, IF, IH(P), WB for the detection of Vimentin. |
| Key Applications |
|
| Application Notes | Western blot Immunohistochemistry: 1:100; Frozen and paraffin-embedded tissue sections with a minimum of a one hour incubation (longer for paraffin); protease pretreatment is recommended for paraffin-embedded sections. Immunofluorescence ELISA Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | Vimentin purified from bovine lens |
| Clone | VIM 3B4 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | Highly specific for the intermediate filament protein vimentin which is present in all cells of mesenchymal origin. This antibody is positive with cells of mesenchymal derivation, including endothelial cells and certain smooth muscle cells of the vascular tract, fibroblasts, connective tissue, all types of blood cells, including thymocytes, interstitial and testicular Sertoli cells, ovarian follicle cells. It can also be used to detect coexpression of vimentin in combination with other intermediate filament proteins. VIM 3B4 has turned out to be the most avid monoclonal antibody to vimentin. Tumors specifically detected: sarcoma (including myosarcoma), lymphoma, melanoma. The binding region of monoclonal antibody VIM3B4 has been characterized by Bohn et al. (1992). According to these authors, the epitope has been localized on the alpha-helical part of vimentin (rod domain coil 2). Due to an aa substitution at position of aa 353 in murine vimentin (that could explain for the weak cross-reaction of the antibody with murine vimentin) they were able to narrow down the binding region around position 353. These findings were confirmed by truncation mutagenesis experiments using human vimentin (Rogers et al., 1995). Polypeptide reacting: MW 57 kDa intermediate filament protein (vimentin) of mesenchymal cells. |
| Isotype | IgG2a |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin (MIM 125660) is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.[supplied by OMIM] |
| Gene Symbol |
|
| Purification Method | Protein A Purfied |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P08670 # Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. SIZE: 466 amino acids; 53652 Da SUBUNIT: Homopolymer. Component of a complex composed at least of ACTB, AP2M1, AP2A1, AP2A2, MEGF10 and VIM. Interacts with HCV core protein. TISSUE SPECIFICITY: Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitts lymphoma cell lines. Expressed in many hormone- independent mammary carcinoma cell lines. PTM: One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. SIMILARITY: SwissProt: P08670 ## Belongs to the intermediate filament family. |
| Molecular Weight | 57 kDa. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at 2–8°C for up to 12 months from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 50 µg |