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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IHC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | CBL266 |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Anti-Cytokeratin 1 Antibody, 10, clone LH1 |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | The monoclonal is presented at a concentration of 100μg/1ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect Cytokeratin 1 using this Anti-Cytokeratin 1 Antibody, 10, clone LH1 validated for use in WB, IH. |
| Key Applications |
|
| Application Notes | Western blotting. IHC formalin fixed sections, HIER (Microwave citrate buffer pH6.0) produces some staining but pretreatment with type XXIV protease (0.5mg/ml) produces best results. Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | Keratin 1 and 10. |
| Clone | LH1 |
| Host | Mouse |
| Specificity | This antibody reacts with the suprabasal cells of stratified squamous epithelia in human, murine and porcine species. |
| Isotype | IgG1 |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | This gene encodes a member of the type I (acidic) cytokeratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. Mutations in this gene are associated with epidermolytic hyperkeratosis. This gene is located within a cluster of keratin family members on chromosome 17q21. |
| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | SIZE: 644 amino acids; 66018 Da SUBUNIT: Heterotetramer of two type I and two type II keratins. keratin-1 is generally associated with keratin-10. TISSUE SPECIFICITY: The source of this protein is neonatal foreskin. The 67-kDa type II keratins are expressed in terminally differentiating epidermis. PTM: Undergoes deimination of some arginine residues (citrullination). DISEASE: SwissProt: P04264 # Defects in KRT1 are a cause of epidermolytic hyperkeratosis (EHK) [MIM:113800]; also known as bullous congenital ichthyosiform erythroderma (BIE). EHK is a hereditary skin disorder characterized by blistering and a marked thickening of the stratum corneum. & Defects in KRT1 are the cause of Curth-Macklin type ichthyosis hystrix (IHCM) [MIM:146590]. & Defects in KRT1 are a cause of nonepidermolytic palmoplantar keratoderma (NEPPK) [MIM:600962]; also known as Unna- Thost disease. & Defects in KRT1 are a cause of annular epidermolytic ichthyosis (AEI) [MIM:607602]; also known as cyclic ichthyosis with epidermolytic hyperkeratosis. AEI resembles clinical and histologic features of both epidermolytic hyperkeratosis and ichthyosis bullosa of Siemens. & Defects in KRT1 are the cause of keratosis palmoplantaris striata III (PPKS3) [MIM:607654]; also known as striate palmoplantar keratoderma III (SPPK3). PPKS3 is an autosomal dominant disorder affecting palm and sole skin where stratum corneum and epidermal layers are thickened. There is no involvement of non-palmoplantar skin, and both hair and nails are normal. SIMILARITY: SwissProt: P04264 ## Belongs to the intermediate filament family. MISCELLANEOUS: There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa). |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | For use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |