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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | FC, IF, IHC, ICC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | CBL271 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Fibroblasts Antibody, clone TE-7 |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | The monoclonal is presented at a concentration of 100 μg/mL in phosphate buffered saline containing 10 mM sodium azide and 1 mg/mL bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Fibroblasts Antibody, clone TE is validated for use in Flow Cytometry (FC), Immunocytochemistry (ICC), Immunofluorescence (IF) and Immunohistochemistry (IHC) for the detection of Fibroblasts. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A representative lot immunostained fibroblastic cells in paraffin-embedded gastrocnemius section from a patient with myopathic condition (Courtesy of Duy Minh Ha, University of Nebraska Medical Center, Omaha, NE) Immunocytochemistry Analysis: A representative lot immunostained human muscle fibroblasts by fluorescent immunocytochemistry (Agley, C.C., et al. (2015). J. Vis. Exp. (95):52049). Immunocytochemistry Analysis: A representative lot immunostained MRC-5 fetal fibroblasts and normal human dermal fibroblasts (NHDF), but not fibrocytes or macrophages by immunocytochemistry (Bertolotto, C., et al. (2009). PLoS One. 4(10):e7475). Flow Cytometry Analysis: A representative lot detected fibroblasts differentiated from human ESCs and iPSCs (Zhang, J., et al. (2012). Circ. Res. 111(9):1125-1136). Immunofluorescence Analysis: A representative lot immunostained fibroblasts in human skeletal muscle cryosections by fluorescent immunohistochemistry (Agley, C.C., et al. (2013). J. Cell. Sci. 126(Pt 24):5610-5625). Immunofluorescence Analysis: A representative lot immunostained the fibrous stroma and vessels in acetone-fixed, frozen human tissue sections by fluorescent immunohistochemistry (Haynes, B.F., et al. (1984). J. Exp. Med. 159(4):1149-1168). |
| Biological Information | |
|---|---|
| Immunogen | Whole human thymic stoma cells (Haynes, B.F., et al. (1984). J. Exp. Med. 159(4):1149-1168). |
| Clone | TE-7 |
| Host | Mouse |
| Specificity | The antibody reacts with fibroblasts in tissue as well as cultured fibroblasts. On skin sections the antibody reacts with stratum basal epidermal cells. The antigen defined by antibody TE-7 appears to be a marker for mesodermally dervied (mesenchymal) human connective tissue and tumors derived from such tissue, e.g. fibrosarcomas (Haynes, B.F., et al. (1984). J. Exp. Med. 159(4):1149-1168). The antibody is useful for the assessment of thymic epithelial culture contamination with fibroblasts, and the identification of cells of mesodermal origin in the fetus. |
| Isotype | IgG1 |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Product Usage Statements | |
|---|---|
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | For use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |