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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | FC, IHC, IH(P), IP | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | CBL468 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-PECAM-1 Antibody, domains 3-6 of human PECAM-1, clone HC1/6 |
| Alternate Names |
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| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-PECAM-1 Antibody, domains 3-6 of human PECAM-1, clone HC1/6 is an antibody against PECAM-1 for use in FC, IH, IH(P) & IP. |
| Key Applications |
|
| Application Notes | Studies of the role of CD31 in leucocyte migration into inflammatory sites and in the process of angiogenesis This antibody will stain frozen tissue sections Suitable for immunoprecipitation studies Suitable for flow cytometry either indirectly or directly when labelled with FITC or R-PE Suitable for use on formalin fixed paraffin embedded sections after protease pre-treatment. Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | PMA - treated U937 cells |
| Epitope | domains 3-6 of human PECAM-1 |
| Clone | HC1/6 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | Reacts with a 130-140 kDa transmembranous glycoprotein that is expressed widely amongst cells of the vascular compartment. CD31 is expressed on platelets, cultured endothelial cells, monocytes, normal tissue macrophages in tonsil, lung and kidney as well as in skin biopsies from conditions such as sarcoidosis and lepromatous leprosy. FUSION PARTNER: P3-x63Ag8.653 myeloma cell line |
| Isotype | IgG1 |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
|
| Purification Method | Protein A Purfied |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P16284 # This protein is a cell adhesion molecule expressed on platelets and at endothelial cell intercellular junctions. SIZE: 738 amino acids; 82536 Da SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. TISSUE SPECIFICITY: Long isoform predominates all tissues examined, isoform Delta12 was detected only in trachea and isoform Delta14-15 only in lung, isoform Delta14 was detected in all tissues examined with the strongest expression in heart. PTM: Phosphorylated on Ser and Tyr residues after cellular activation. SIMILARITY: SwissProt: P16284 ## Contains 6 Ig-like C2-type (immunoglobulin-like) domains. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | For use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C for up to one year. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |