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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | FC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | CBL555 |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Anti-CD34 Class III Antibody, clone 581 |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Protein G affinity chromatography. The monoclonal is presented at a concentration of 100μg/1ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-CD34 Antibody, Class III, clone 581 is validated for use in FC for the detection of CD34 Class III. |
| Key Applications |
|
| Application Notes | Study of the Class III CD34 epitope by FACS Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Clone | 581 |
| Host | Mouse |
| Specificity | Clone 581 reacts with the class III CD34 epitope is resistant to neuraminidase, chymopapain and glycoprotease. The antibody reacts with a single-chain 105-120 kDa heavily O-glycosylated transmembrane glycoprotein which is expressed on haematopoietic progenitor cells, vascular endothelium and some tissue fibroblasts. The intracellular chain of the CD34 antigen is a site of phosphorylation by activated protein kinase C suggesting a putative role in signal transduction. CD34 may also play a role in the adhesion of specific antigens to endothelium. |
| Isotype | IgG1 |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | CD34 is a monomeric cell surface antigen with a molecular mass of approximately 110 kD that is selectively expressed on human hematopoietic progenitor cells.[supplied by OMIM] |
| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P28906 # Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins. SIZE: 385 amino acids; 40716 Da SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. TISSUE SPECIFICITY: Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues. DEVELOPMENTAL STAGE: On early hematopoietic progenitor cells. PTM: Highly glycosylated. & Phosphorylated on serine residues by PKC. DISEASE:SwissProt: P28906 # Abnormal CD34 expression in leukemogenesis. SIMILARITY: SwissProt: P28906 ## Belongs to the CD34 family. |
| Product Usage Statements | |
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| Availability by Geography |
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| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | For use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |