| Description | |
|---|---|
| Catalogue Number | CC1018 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Human Integrin αVβ3 Protein, Triton X-100 Formulation |
| Overview | Integrin alphaVbeta3 was purified from human placenta by affinity chromatography using immobilized monoclonal antibodies to alphaVbeta3 integrin. A tissue detergent extract, which was applied to the column was prepared as previously described [Belkin, 1990]. Two main protein bands corresponding to alphaV (145 kDa) and beta3 (92 kDa) subunits are seen in silver stained polyacrylamide gels under non-reducing conditions. Minor protein band (170 kDa) corresponds to beta3 subunit disulfide dimer. |
| Product Information | |
|---|---|
| Presentation | Purified protein in 20 mm Tris-HCI, pH 7.5, 150 mm NaCI, 2 mm MgCI2, 0.2% Triton X-100. No preservatives. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Key Applications |
|
| Application Notes | Electrophoresis and Immunoblotting control. Also useful for in vitro binding experiments with metalloproteinase MMP-2 . |
| Biological Information | |
|---|---|
| Concentration | 200 μg/mL |
| Purity | >95% by gel analysis 7.5% SDS-PAGE by the method of Laemmli gives two major protein bands, corresponding to alpha V subunit (145 kDa) and beta 3 subunit (92 kDa) when visualized by silver stain (2-2.5 mg protein / lane nonreduced). A minor protein band (170 kDa) corresponds to the beta 3 dimer, similar to an occurrence of the beta 1 dimer upon purification of beta 1 integrins [Belkin et Al., 1996]. In some cases a beta 3 trimer is visible at 260 kDa. After reduction, with protein quantities of 1.5 - 4μg protein / lane, three major protein bands are apparent, corresponding to beta 3 integrin subunit (110 kDa), heavy alpha V chain (125 kDa) and light alpha V chain (25 kDa). A minor band at 200 kDa region is presumed to be the reduced beta 3 dimer, consistent with previous publications describing a 230 kDa beta 1 reduced dimer for beta 1 integrin purifications described previously. [Belkin et Al., 1996]. Faint bands at 80 kDa and 55 kDa correspond to minor degradation products of beta 3 . CONTAMINANTS: Two faint bands identified at 210 and 220 kDa, which may correspond to the alpha V dimer. |
| Specific Activity | Functional activity confirmed by interaction of integrin alphaV/beta3 with vitronectin, von Willebrand Factor, fibrinogen, and fibronectin in RGD-dependent ELISA testing. |
| Entrez Gene Number |
|
| Entrez Gene Summary | ITAGV encodes integrin alpha chain V. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. The I-domain containing integrin alpha V undergoes post-translational cleavage to yield disulfide-linked heavy and light chains, that combine with multiple integrin beta chains to form different integrins. Among the known associating beta chains (beta chains 1,3,5,6, and 8; ITGB1, ITGB3, ITGB5, ITGB6, and ITGB8), each can interact with extracellular matrix ligands; the alpha V beta 3 integrin, perhaps the most studied of these, is referred to as the Vitronectin receptor (VNR). In addition to adhesion, many integrins are known to facilitate signal transduction. |
| Gene Symbol |
|
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P06756 # The alpha-V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and von Willebrand factor. They recognize the sequence R-g-D in a wide array of ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposis sarcoma lesions. SIZE: 1048 amino acids; 116038 Da SUBUNIT: Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of an heavy and a light chain linked by a disulfide bond. Alpha-V associates with either beta-1, beta-3, beta-5, beta-6 or beta-8 subunit. Interacts with HIV-1 Tat. SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. SIMILARITY: SwissProt: P06756 ## Belongs to the integrin alpha chain family. & Contains 7 FG-GAP repeats. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at -70°C in undiluted aliquots. Avoid repeated freeze/ thaw cycles. |
| Packaging Information | |
|---|---|
| Material Size | 25 µg |