| Description | |
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| Catalogue Number | CC1055 |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Human Integrin α5β1, Triton X-100 Formulation |
| Overview | Integrin a5/b1 purified from smooth muscle and placental tissue under nondenaturing conditions by affinity chromatography on fibronectin-sepharose (Belkin, 1990). Two main protein bands corresponding to a5 (145 kD) and beta1 (120 kD) subunits are seen in silver stained 7.5% polyacrylamide gels under nonreduced conditions. Minor protein band at the 210 kD corresponding to beta1 subunit disulfide dimmer, which is formed during electrophoresis and disappears after reduction. |
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| Product Information | |
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| Presentation | Purified protein in 20 mM Tris-HCl, pH 7.5, 150 mM NaCl, 2 mM MgCl2 containing 0.2% Triton X-100. No preservatives. Source material for the enclosed purified human proteins, Integrin alpha5beta1, has been tested for antibodies to HIV, HCV, and HbsAg and found to be negative by an approved test. |
| Quality Level | MQ100 |
| Applications | |
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| Key Applications |
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| Application Notes | Electrophoresis and Immunoblotting control. Due to the existance of reduction sensitive sites within the integrin subunits, it is recommended that gels be run under non-reducing conditions for best results. Under non-reducing conditions, the alpha5 subunit will migrate at 155 kDa while the beta1 subunit will migrate at 110 kDa. Function: Liposomes containing Integrin alpha5/beta1 adhere to surfaces coated with fibronectin in an RGD-dependent fashion. |
| Biological Information | |
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| Purity | Nonreduced SDS-PAGE of 15 μg on 5-15% polyacrylamide slab gel and staining with Coomassie shows only two main bands at 110 kDa (beta1 subunit) and 155 kDa (alpha5 subunit). A weak band at 205 kDa is due to dimerization of the b1 subunit (Belkin et al., 1996). CONTAMINANT(S): None identified. |
| Source | Human smooth muscle and placental tissue. |
| Entrez Gene Number |
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| Entrez Gene Summary | The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling. |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P08648 # Integrin alpha-5/beta-1 is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposis sarcoma lesions. SIZE: 1049 amino acids; 114536 Da SUBUNIT: Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of an heavy and a light chain linked by a disulfide bond. Alpha-5 associates with beta-1. Interacts with HPS5. Interacts with HIV-1 Tat. SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. SIMILARITY: SwissProt: P08648 ## Belongs to the integrin alpha chain family. & Contains 7 FG-GAP repeats. |
| Product Usage Statements | |
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| Storage and Shipping Information | |
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| Storage Conditions | Maintain at -70°C in undiluted aliquots. Avoid repeated freeze/thaw cycles. |
| Packaging Information | |
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| Material Size | 50 µg |