Key Specifications Table
| Species Reactivity | Key Applications | Detection Methods |
|---|---|---|
| M, Rb | ICC, IHC, IH(P) | Chromogenic |
| Description | |
|---|---|
| Catalogue Number | DAB500 |
| Replaces | DAB250 |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | IHC Select® HRP/DAB, 500 Tests |
| Overview | Summary and Explanation: Immunohistochemical techniques have been in use as adjunctive methods for the recognition of cells in tissue sections for at least 50 years. The first method reported use of fluorescent labels in 19421. In 1974, reports of using enzymatic labels, such as horseradish peroxidase (HRP) in routine H & E paraffin sections appeared2,3. The methods have since become the standard of care in surgical pathology when classic methods alone fail to yield a definitive diagnosis4-9. Early techniques were based on the peroxidase-anti-peroxidase (PAP) reaction28, while improvements exploited the strong affinity of avidin for biotin10. This technique uses HRP-labeled streptavidin. The secondary antibody is conjugated with biotin and the streptavidin-HRP complex reacts with the biotin on the secondary antibodies. The resulting biotin-avidin-HRP complex can react with the primary antibody bound to the specific epitope of the target antigen. The HRP enzymes of the streptavidin complex then catalyze the substrate/chromogen reaction to form a colored reaction product (brown to black when using DAB as the chromogen) at the antigen site. A biological stain is then used to visualize the whole tissue section. |
| Product Information | |
|---|---|
| Components |
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| Detection method | Chromogenic |
| Applications | |
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| Key Applications |
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| Application Notes | The IHC Select™ Immunoperoxidase Secondary Detection System is intended for use with the CHEMICON IHC Select™ Prediluted Primary Antibody reagents which contain rabbit and mouse IgG for the qualitative identification of antigens by light microscopy in paraffin-embedded tissues. The IHC Select™ Immunoperoxidase Secondary Detection System may also be used with other rabbit or mouse IgG primary antibodies. General Purpose Reagents |
| Biological Information | |
|---|---|
| Species Reactivity |
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| Product Usage Statements | |
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| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | Stability and Storage: When stored at 2 - 8°C, the IHC Select™ Immunoperoxidase Secondary Detection System is stable up to the expiration date printed on the kit label. Do not freeze or expose to elevated temperatures. Discard any remaining reagents after the expiration date. Do not store kit components or perform staining in strong light, such as direct sunlight. Precautions : · Pooling, substitution or alteration of any reagent may cause erroneous results. * Do not mix reagents from different lots. * Do not allow the slides to dry at any time during the staining procedure. * Handle all specimens and materials coming in contact with them as potentially infectious materials. Disinfect with 0.05% sodium hypochlorite. * Do not mouth pipette reagents. If ingested, seek professional assistance or contact a Poison Control Center immediately. · Diaminobenzidine (DAB) may be carcinogenic. This solution may cause irritation upon skin contact. Avoid inhalation, ingestion, or skin contact and wear gloves when handling DAB. If skin contact occurs, flush affected area with water. Spills may be neutralized with bleach25,26. Dispose of according to local regulations. · Inhalation or ingestion of xylene or the highly allergenic fixative formaldehyde is harmful. These materials should be handled with gloves. Prepare in a fume hood. If skin or eye contact occurs, wash thoroughly with copious amounts of water. · Wear disposable gloves when handling any human material and while performing the staining procedure. Materials Required: · Primary rabbit or mouse IgG antibodies (such as CHEMICON IHC Select™Prediluted Primary Antibodies) · Positive and negative tissue control slides · Positively charged microscope slides · Glass cover slips (24 x 40mm, No. 1) · Distilled or deionized water · Tween® 20 · Permanent mounting medium, Eukitt® American Histology or equivalent · 3% laboratory grade hydrogen peroxide · 10.0% neutral buffered formalin15 · Xylene, ACS grade · Ethanol, absolute (100%) · Latex, rubber or 100% nitrile gloves (xylene resistant) · Timer · Fume hood · Light microscope equipped with Plan Achromat or equivalent lenses · 60°C drying oven |
| Packaging Information | |
|---|---|
| Material Size | 1 kit |
| Material Package | 500 tests |