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Millipore 10 mL Dab 50x Concentrate, Brown, Horseradish Peroxidase Substrate (Insoluble In Alcohol) For Western Blotting And Immunohistochemistry. - Mill (Additional S&H or Hazmat Fees May Apply)
DAB 50x Concentrate, Brown, Horseradish Peroxidase Substrate (insoluble in alcohol)
Overview
Since first introduced by Graham and Karnovsky (1966), numerous procedures for its use in histochemistry, immunohistochemistry, Western and DOT blots have been described (Lewis & Knight, 1977; Larsson, 1988; Gallyas et Al., 1982). In the presence of horseradish peroxidase and hydrogen peroxide, DAB oxidatively polymerizes to an insoluble brown polymer. The color can be modified and intensified by treatment with metal salts of silver, copper, nickel, cobalt and osmium (Hsu & Soban, 1982). DAB is a suspected carcinogen and must be handled with caution. CHEMICON supplies DAB as a stable liquid concentrate eliminating the necessity of handling a potentially hazardous material. The stabilization system is unique and prevents formation of partially oxidized DAB excluding nonspecific binding to other heme containing proteins SO often observed with powdered DAB preparations. The concentrate can be diluted in appropriate peroxide containing buffers providing the researcher with the capability of formulating any of the numerous published DAB reaction systems. The most common procedure suggests diluting the DAB 1:50 with Chemicon Catalog Number ES010 (Tris-HCl buffer, pH 7.6), and adding H(2)O(2), to a final concentration of 0.01%. Incubation times range from 5-15 minutes. Prolonged reaction time will result in non-specific staining of other heme proteins.
Product Information
Presentation
DAB (50X) concentrate in proprietary solvents and stabilizers.
Color Code
Black
Applications
Application
10 mL DAB 50x Concentrate, Brown, Horseradish Peroxidase Substrate (insoluble in alcohol) for Western Blotting and Immunohistochemistry.
Key Applications
Western Blotting
Immunohistochemistry
Product Usage Statements
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Maintain refrigerated at 2-8°C for up to 6 months after date of receipt. DAB is a suspected carcinogen.
