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EmbryoMax PMEF feeder cells, Millipores convenient solution for ES cell culture.
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EmbryoMax PMEF feeder cells, Millipores convenient solution for ES cell culture.
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Key Specifications Table
| Key Applications | Purity |
|---|---|
| Cult | The active component mLIF has been shown to be >95% pure by SDS-PAGE. ESGRO® supplied 0.22 micron sterile filtered, and tested negative in mycoplasma tests. |
| Description | |
|---|---|
| Catalogue Number | ESG1106 |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | ESGRO® Recombinant Mouse LIF Protein |
| Overview | The ESGRO mouse LIF medium supplement is supplied in active units/mL for consistent performance from lot to lot. Each lot of ESGRO supplement undergoes stringent quality control testing to ensure reliable inhibition of mouse pluripotent stem cell differentiation. ESGRO mouse LIF supplement may be used for feeder based and feeder free culture of mouse embryonic stem (ES) and induced pluripotent stem (iPS) cells. |
| Alternate Names |
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| Background Information | Mouse Leukemia Inhibitory Factor (LIF, mLIF) promotes self-renewal and long-term maintenance of pluripotent mouse embryonic stem (ES) and induced pluripotent stem (iPS) cells by suppressing spontaneous differentiation. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | ESGRO® Supplement is supplied in liquid form as 106 Units in 1.0 mL of phosphate buffered saline with 1% w/v bovine serum albumin BSA as a carrier for stability. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | ESGRO Leukemia Inhibitory Factor (LIF) supplement for mouse ES cell culture. Each vial contains 10^6 units/ml |
| Key Applications |
|
| Application Notes | Applications for ESGRO® Supplement include use as a reagent for the in vitro maintenance of the pluripotential phenotype of murine ES cells. SUGGESTED PROTOCOLS: ES Cells: In D3 and MBL-1 pluripotential ES cell cultures it is routinely found that 1000 U of ESGRO® Supplement per 1.0 mL of tissue culture media is required to maintain ES cells with a stem cell phenotype. Similar concentrations of mLIF have also been used for germline transmission of genetically altered ES cells (E3). At the recommended concentration 107 units of ESGRO® Supplement is sufficient for 10.0 L of tissue culture media and 106 units of ESGRO® Supplement is sufficient for 1.0 L of tissue culture media. |
| Biological Information | |
|---|---|
| Concentration | 1 million units/1 mL |
| Purity | The active component mLIF has been shown to be >95% pure by SDS-PAGE. ESGRO® supplied 0.22 micron sterile filtered, and tested negative in mycoplasma tests. |
| Specific Activity | ESGRO® Supplement is assessed both on mouse embryonic stem cells (D3 & MBL-1) and on murine M1 myeloid leukemic cells(4). A standard of 50 Units is defined as the concentration of ESGRO® Supplement in 1.0mL of tissue culture medium that induces the differentiation of 50% of M1 colonies(4). 10<sup>6</sup> units is equivalent to approximately 10 μg of pure protein, and is sufficient to treat 1L of ES cell culture media. <br /><br />Embryonic Stem Cell Assay: Differentiation inhibition at 1000 units/mL Murine myeloid leukemic, M1 Assay: Specific Activity >/= 10<sup>8</sup> units/mg |
| Entrez Gene Number |
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| Entrez Gene Summary | Leukaemia inhibitory factor is a cytokine that induces macrophage differentiation. Neurotransmitters and neuropeptides, well known for their role in the communication between neurons, are also capable of activating monocytes and macrophages and inducing chemotaxis in immune cells. LIF signals through different receptors and transcription factors. LIF in conjunction with BMP2 acts in synergy on primary fetal neural progenitor cells to induce astrocytes. |
| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P15018 # LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes. SIZE: 202 amino acids; 22008 Da SUBCELLULAR LOCATION: Secreted. SIMILARITY: SwissProt: P15018 ## Belongs to the LIF/OSM family. |
| Media Form | Liquid |
| Stem Cell Type |
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| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | ESGRO® Supplement is shipped at ambient temperature. A cold pack may be included for customer relations, however it is truly not needed. Extensive stability tests have been performed with ESGRO® Supplement during development. This product is stable for at least 18 months from the date of manufacture, in the concentrated form or diluted in sterile tissue culture media, with no loss of activity on ES cells. For long term storage it is recommended that ESGRO® Supplement be stored at 4°C. Freeze-thawing will reduce potency. It is recommended that prior to use, ESGRO® Supplement should be diluted in sterile tissue culture media and aliquoted to a convenient concentration, then stored at 4°C. Freeze-thawing should be avoided. Product is stable for a minimum of 7 days at 37°C, 5% CO2 incubator during the culture of ES cells. |
| Packaging Information | |
|---|---|
| Material Size | 10⁶ units |
| Material Package | 1 mL |