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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | FC, ICC | M | AlexaFluor®488 | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | FCMAB113A4 |
| Description | Anti-Oct-4 Antibody, clone 10H11.2, Alexa Fluor® 488 conjugate |
| Alternate Names |
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| Background Information | Octamer-4 (Oct-4), a member of the POU family of transcription factors, has been demonstrated to be vital for the formation of self-renewing pluripotent stem cells. During embryogenesis expression of Oct-4 is limited to pluripotent cells of the inner cell mass (ICM) that contribute to the formation of all fetal cell types. This relationship between Oct-4 and pluripotency has seen this transcription factor emerge as a marker of pluripotent stem cells. Undifferentiated human and murine pluripotent embryonic stem (ES) and embryonic carcinoma (EC) cells express Oct-4. Additionally, murine embryonic germ (EG) cells are also known to express Oct-4. Following stem cell differentiation, the level of Oct-4 expression decreases. Oct-4 expression by human EG cells is not known. |
| Product Information | |
|---|---|
| Format | AlexaFluor®488 |
| Control |
|
| Presentation | Purified mouse monoclonal IgG1 conjugated to Alexa Flour® 488 in PBS with less than 0.09% sodium azide and 15 mg/mL BSA. |
| Quality Level | MQ100 |
Antibody dilution for cellular staining:
• Prepare an antibody working solution by diluting 1:5 the primary antibody with PBS.
• Dispense the volume per test of working of solution according to the number of cells indicated in the table below.
• 5 µL for Guava Flow Cytometer
• 10 µL for other Flow Cytometry instruments
| Applications | |
|---|---|
| Application | Detect Oct-4 using this Anti-Oct-4 Antibody, clone 10H11.2, Alexa Fluor 488 conjugate validated for use in FC & IC. |
| Key Applications |
|
| Working Solution | Cells / test | Total Reaction Volume |
|---|---|---|
| 5 μl | 5 x 105 Cells / 95 μl PBS | 100 μl |
| 10 μl | 1 x 106 Cells / 90 μl PBS | 100 μl |
| Biological Information | |
|---|---|
| Immunogen | Recombinant GST fusion protein, human Oct-4 (aa 1-134). |
| Clone | 10H11.2 |
| Host | Mouse |
| Specificity | Reacts with human Oct-4. |
| Isotype | IgG1 |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION:Transcription factor that binds to the octamer motif (5-ATTTGCAT-3). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency By similarity. SUBUNIT STRUCTURE:Interacts with UBE2I By similarity. Interacts with PKM2. SUBCELLULAR LOCATION: Nucleus. Note: Expressed in a diffuse and slightly punctuate pattern. TISSUE SPECIFICITY: Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues. INDUCTION: Transcriptional activity is positively regulated by PKM2. DOMAIN: The POU-specific domain mediates interaction with PKM2. PTM: Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression By similarity. MISCELLANEOUS: Several pseudogenes of POU5F1 have been described on chromosomes 1, 3, 8, 10 and 12. 2 of them, localized in chromosomes 8 and 10, are transcribed in cancer tissues but not in normal ones and may be involved in the regulation of POU5F1 gene activity in carcinogenesis. SEQUENCE SIMILARITIES:Belongs to the POU transcription factor family. Class-5 subfamily. Contains 1 homeobox DNA-binding domain.Contains 1 POU-specific domain. |
| Molecular Weight | ~44 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Flow Cytometry in 2102Ep Cells. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain refrigerated at 2-8°C protected from light in undiluted aliquots for up to 6 months from date of receipt |
| Packaging Information | |
|---|---|
| Material Size | 100 tests |