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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | FC | M | FITC | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | FCMAB191F |
| Trade Name |
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| Description | Milli-Mark™ Anti-CD27-FITC Antibody, clone M-T271 |
| Alternate Names |
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| Background Information | CD27, a member of the nerve growth factor receptor (NGFR) superfamily, is a type I transmembrane protein consisting of a disulphide-linked 120 kDa homodimer (Bigler, 1988; Sugita, 1992; Morimoto, 1995). CD27 is expressed on mature thymocytes and on the majority of human peripheral blood T cells, on both CD4+ and CD8+ subsets. CD27 is also expressed on activated B cells and a fraction of resting natural killer (NK) cells (Maurer, 1990; Sugita, 1992). Among CD4+ T cells, CD27 is preferentially expressed on unprimed CD4+CD45RA+CD45R0- T cells, while primed CD4+CD45RA-CD45R0+ T cells express low levels of CD27 (Sugita, 1992). During activation, the expression of CD27 is increased on B cells and unprimed T cells, while primed T cells have a relatively low capacity for upregulation of CD27 (Morimoto, 1995; Hintzen, 1994). The activation is also accompanied by the appearance of a 32 kDa soluble form of CD27 (sCD27) found in supernatants of activated lymphocytes (Hintzen, 1994). CD27 is believed to play a role in T cell activation, since the expression is upregulated during T cell activation (Bigler, 1988; Sugita, 1992; Morimoto, 1995; Maurer, 1990; Hintzen, 1994; Lens, 1995). |
| Product Information | |
|---|---|
| Format | FITC |
| Control |
|
| Presentation | Purified Mouse monoclonal IgG1 conjugated to FITC in 0.05M Tris-HCl buffer with 1% BSA, Sodium Azide at 0.097% at pH 7.2. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Milli-Mark Anti-CD27-FITC Antibody, clone M-T271 is validated for use in FC for the detection of CD27. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | Leukemic peripheral blood T cells from a patient with chronic T cell leukemia (T-CLL) |
| Clone | M-T271 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | Antibody recognizes Human CD27 antigen. |
| Isotype | IgG1κ |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Purification Method | Protein A Purfied |
| UniProt Number |
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| Molecular Weight | 120 kDa (Bigler, 1988; Sugita, 1992; Morimoto, 1995) |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by flow cytometry using human PBMCs |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintained refrigerated at 2-8°C from date of receipt. Protected from light in undiluted aliquots for 6 months from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 tests |