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Millipore Milli-Mark Anti-Cd33-Fitc Antibody, Clone Wm-54 Detects Level Of Cd33 & Has Been Published & Validated For Use In Fc. - Mill (Additional S&H or Hazmat Fees May Apply)
CD33 is a member of the siglec family (sialic acid binding immunoglobulin-like lectins) referred to as Siglec-3 (Freeman SD, Kelm S, Barber EK and Crocker PR, 1995, Blood, 2005-2012). It is the smallest member of the siglec family having a molecular weight of 67 kDa (Peiper SC, Ashmun RA and Look TA (1988) Blood., 72: 314-321). CD33 mediates protein-carbohydrate cellular interactions in addition to protein-protein recognition (Crocker, 2002). The CD33 gene is located on chromosome 19 (Peiper, 1997). CD33 is expressed by subsets of monocytes and neutrophils. CD33 is the major antigen to differentially diagnose myeloid from lymphoid leukemias, as it is expressed on 90% of myeloid and not lymphoid leukemic cells (Andrews 1989; Griffin 1984; and Sabbath 1986). Committed hemapoietic progenitors, including multipotential stem cells (CFU-GEMM) and precursors of granulocytes and macrophages (CFU-GM) and early erythroid progenitors (BFU-E) express CD33 (Peiper, 1989 and 1997). Anti-CD33, WM-54, was included in the Fourth International Workshop and Conference on Human Leucocyte Differentiation Antigens and studies by a number of laboratories confirmed its reactivity with CD33 (Peiper, 1989).
Product Information
Format
FITC
Control
PBMCs
Presentation
Purified Mouse monoclonal IgG1 conjugated to FITC in 0.05M Tris-HCl buffer with 1% BSA, Sodium Azide at 0.097% at pH 7.2.
Quality Level
MQ100
Applications
Application
Milli-Mark Anti-CD33-FITC Antibody, clone WM-54 detects level of CD33 & has been published & validated for use in FC.
Key Applications
Flow Cytometry
Biological Information
Immunogen
Leukemic cells from a patient with AML.
Clone
WM-54
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Specificity
Antibody recognizes Human CD33 antigen.
Isotype
IgG1κ
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_001763.3
Purification Method
Protein A Purfied
UniProt Number
P20138
Molecular Weight
67 kDa (Peiper, 1997)
Product Usage Statements
Quality Assurance
Evaluated by flow cytometry using human PBMCs
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Maintained refrigerated at 2-8°C from date of receipt. Protected from light in undiluted aliquots for 6 months from date of receipt.
