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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | FC | M | FITC | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | FCMAB194F |
| Trade Name |
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| Description | Milli-Mark™ Anti-CD38-FITC Antibody, clone AT13/5 |
| Alternate Names |
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| Background Information | CD38 is a 45 kDa type II integral membrane glycoprotein consisting of an N-terminal 20 amino acid cytoplasmic domain, a 23 amino acid transmembrane domain, and a 257 amino acid extracellular domain. CD38 is associated with different molecules in different cell types and these unique combinations cause unique cell-type specific signaling. One of the known ligands that activates CD38-mediated signaling is CD31 (Deaglio, 2001). CD38 is expressed by a number of different cell types and the pattern of expression often changes with differentiation and maturation. Thus, CD38 expression is found during early stages of B and T-cell differentiation, is lacking during the later intermediate stages, and is regained near the end of maturation. CD38 is expressed at variable levels on the majority of hematopoietic cells, prevalently during early differentiation and activation, and it is expressed strongly on plasma cells (Jackson, 1990; Terstappen, 1990; Morra, 1997). CD38-postive cell types outside the hematopoietic system include subsets from the gut, brain, prostate, pancreas, bone, eye, and muscle (Deaglio, 2001). CD38 is expressed on approximately 60% of CD34-positive peripheral blood mononuclear cells. The least mature CD34-postive cells are characterized by a lack of CD38 (Herbein, 1994). Anti-CD38, AT13/5 was included in the Sixth International Workshop and Conference on Human Leucocyte Differentiation Antigens, and studies by a number of laboratories confirmed its reactivity with CD38 (Morra, 1997). |
| Product Information | |
|---|---|
| Format | FITC |
| Control |
|
| Presentation | Purified Mouse monoclonal IgG1 conjugated to FITC in 0.05M Tris-HCl buffer with 1% BSA, Sodium Azide at 0.097% at pH 7.2. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Milli-Mark Anti-CD38-FITC Antibody, clone AT13/5 is an antibody against CD38 for use in FC. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | Namalwa cells (B lymphocyte cell line from Burkitts lymphoma) |
| Clone | AT13/5 |
| Host | Mouse |
| Specificity | Antibody recognizes Human CD38 antigen. |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | CD38 is a novel multifunctional ectoenzyme widely expressed in cells and tissues especially in leukocytes. CD38 also functions in cell adhesion,signal transduction and calcium signaling. |
| Purification Method | Protein A Purfied |
| UniProt Number |
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| UniProt Summary | Synthesizes cyclic ADP-ribose, a second messenger for glucose-induced insulin secretion. Also has cADPr hydrolase activity. Also moonlights as a receptor in cells of the immune system. Expressed at high levels in pancreas, liver, kidney, brain, testis, ovary, placenta, malignant lymphoma and neuroblastoma. Preferentially expressed at both early and late stages of the B and T-cell maturation. It is also detected on erythroid and myeloid progenitors in bone marrow, where the level of surface expression was shown to decrease during differentiation of blast-forming unit E to colony-forming unit E. |
| Molecular Weight | 45 kDa (Deaglio, 2001) |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by flow cytometry using human lymphocytes |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain refrigerated at 2-8°C protected from light in undiluted aliquots for up to 6 months from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 tests |