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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | FC | M | FITC | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | FCMAB207F |
| Trade Name |
|
| Description | Milli-Mark™ Anti-CD71-FITC Antibody, clone Ber-T9 |
| Alternate Names |
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| Background Information | CD71 is a 190 kDa disulfide-bonded homodimeric type II transmembrane glycoprotein. CD71 is the receptor for transferrin. CD71 is involved in iron (Fe3+) uptake, and expression is regulated by the metabolic demand for iron, which is essential for the growing cell (Goding, 2002). CD71 is more abundantly expressed in rapidly dividing cells than quiescent cells (Newman, 1982; Sutherland, 1981). In normal tissues, high expression of CD71 is seen in the placental trophoblast and hemoglobin-synthesizing reticulocytes (Newman, 1982) but expression is lost in mature erythrocytes (Sutherland, 1981). Anti-CD71, Ber-79, was included in the Fifth International Workshop and Conference on Human Leucocyte Differentiation Antigens, and studies by a number of laboratories confirmed its reactivity with CD71 (Boeker, 1995). |
| Product Information | |
|---|---|
| Format | FITC |
| Control |
|
| Presentation | Purified Mouse monoclonal IgG1 conjugated to FITC in 0.05M Tris-HCl buffer with 1% BSA, Sodium Azide at 0.097% at pH 7.2. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect CD71 using this Milli-Mark Anti-CD71-FITC Antibody, clone Ber-T9 validated for use in FC. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Clone | Ber-T9 |
| Host | Mouse |
| Specificity | Antibody recognizes Human CD71 antigen. |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Purification Method | Protein A Purfied |
| UniProt Number |
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| UniProt Summary | Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system By similarity. A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. |
| Molecular Weight | 190 kDa (Goding, 2002) |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by flow cytometry using human lymphocytes |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain refrigerated at 2-8°C protected from light in undiluted aliquots for up to 6 months from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 tests |