Special Offers
100% Performance Guaranteed
Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | FC | M | FITC | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | FCMAB217F |
| Trade Name |
|
| Description | Milli-Mark™ Anti-CD235a-FITC Antibody, clone JC159 |
| Alternate Names |
|
| Background Information | Glycophorin A, CD235a, is a single-pass membrane sialoglycoprotein and carrier of the blood group specificities M and N. The gene is located on chromosome 4q28-q31 and share sequence homology with genes encoding glycophorin B and glycophorin E (van der Schoot, 2002). Glycophorin A is expressed by erythroid cells beginning on morphologically recognizable erythroid precursors, just after the CFU-E stage, to the mature erythrocyte. Once glycophorin A is maximally expressed, the amount in each erythroid cell remains constant and does not appear to change during further maturation (Loken, 1987). |
| Product Information | |
|---|---|
| Format | FITC |
| Control |
|
| Presentation | Purified Mouse monoclonal IgG1 conjugated to FITC in 0.05M Tris-HCl buffer with 1% BSA, Sodium Azide at 0.097% at pH 7.2. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect CD235a using this Milli-Mark Anti-CD235a-FITC Antibody, clone JC159 validated for use in FC. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | Membrane extracts of hairy cell leukemia prepared from human spleen |
| Clone | JC159 |
| Host | Mouse |
| Specificity | Antibody recognizes Human CD235a antigen. |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | Glycophorins A (GYPA) and B (GYPB) are major sialoglycoproteins of the human erythrocyte membrane which bear the antigenic determinants for the MN and SS blood groups. In addition to the M or N and S or s antigens that commonly occur in all populations, about 40 related variant phenotypes have been identified. These variants include all the variants of the Miltenberger complex and several isoforms of Sta, as well as Dantu, Sat, He, mg, and deletion variants Ena, S-s-U- and Mk. Most of the variants are the result of gene recombinations between GYPA and GYPB. |
| Purification Method | Protein A Purfied |
| UniProt Number |
|
| UniProt Summary | Glycophorin A is the major intrinsic membrane protein of the erythrocyte. The N-terminal glycosylated segment, which lies outside the erythrocyte membrane, has MN blood group receptors. Appears to be important for the function of SLC4A1 and is required for high activity of SLC4A1. May be involved in translocation of SLC4A1 to the plasma membrane. Is a receptor for influenza virus. Is a receptor for Plasmodium falciparum erythrocyte-binding antigen 175 (EBA-175); binding of EBA-175 is dependent on sialic acid residues of the O-linked glycans. Appears to be a receptor for Hepatitis A virus (HAV). |
| Molecular Weight | 16 kDa (calculated) |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by flow cytometry using human lymphocytes |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain refrigerated at 2-8°C protected from light in undiluted aliquots for up to 6 months from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 tests |