Key Specifications Table
| Species | Source | Purity |
|---|---|---|
| Human | expressed in E. coli | Greater than 95% by SDS-PAGE. Endotoxin level is less than 0.1 ng per μg of bFGF. |
| Description | |
|---|---|
| Catalogue Number | GF003AF-100UG |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Fibroblast Growth Factor basic, human recombinant, animal-free |
| Alternate Names |
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| Background Information | Fibroblast Growth Factor-basic (bFGF) is a heparin binding growth factor which stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal and endothelial cells. bFGF also exerts a potent angiogenic activity in vivo. Human bFGF is a 17.2 kDa protein containing 155 amino acid residues. Xu, et Al. demonstrated that bFGF synergizes with the BMP antagonist noggin to sustain undifferentiated proliferation of human embryonic stem (hES) cells under feeder-free conditions. GF003AF was developed without animal-based ingredients and can be used for the culture of hES cells in a feeder-free, animal-free culture system. |
| Product Information | |
|---|---|
| Presentation | Lyophilized from a solution of 5mM Tris, pH 7.6 with 150 mm NaCl. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application Notes | For most in vitro applications, bFGF exerts its biological activity in the concentration range of 0.1 to 10.0 ng/mL. Responding cells are (partial list): Endothelial, mesenchymal cells. Human ES cells require concentrations in the range of 4 to 100 ng/mL, depending on the method of culture |
| Biological Information | |
|---|---|
| Purity | Greater than 95% by SDS-PAGE. Endotoxin level is less than 0.1 ng per μg of bFGF. |
| Source | expressed in E. coli |
| Specific Activity | Chemicons Human FGF-b is fully biologically active when compared to standards. The ED<sub>50</sub> was determined by the dose-dependent stimulation of thymidine uptake by NIH3T3 cells expressing FGF receptors. |
| Entrez Gene Number |
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| Entrez Gene Summary | The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. |
| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P09038 # The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors. SIZE: 155 amino acids; 17254 Da SUBUNIT: Monomer. Interacts with CSPG4 and FGFBP1. Found in a complex with FGFBP1, FGF1 and FGF2. SIMILARITY: SwissProt: P09038 ## Belongs to the heparin-binding growth factors family. MISCELLANEOUS: This protein binds heparin more strongly than does aFGF. |
| Stem Cell Type |
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| Product Usage Statements | |
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| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain the lyophilized material at -20°C until expiration date as stated on the label. General applications: After a quick spin, reconstitute in 0.1M phosphate buffer, pH 6.8, to a concentration of 0.1-1.0 mg/mL. Reconstituted bFGF should be stored in working aliquots at -20°C for up to six months. Multiple freeze/thaw cycles will result in significant loss of activity. For Human ES cell culture: After a quick spin, reconstitute to 10 μg/mL in a filtered solution of 0.5% BSA, 1 mm DTT, and 10% glycerol in Dulbeccos PBS. Aliquot and store at -20°C for up to six months. This solution can then thawed and diluted to 4 ng/mL for the culture of human ES cells with a feeder layer, or to 8 ng/mL to supplement mouse embryonic fibroblast-conditioned medium (for feeder-free human ES cell culture). |
| Packaging Information | |
|---|---|
| Material Size | 100 ug |