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Millipore Human Trail (Tnf-Related Apoptosis Inducing Ligand), Also Called Apo2 Ligand, Is A Cytotoxic Protein Which Activates Rapid Apoptosis In Tumor Cells, But Not In Normal Cells. - Mill (Additional S&H or Hazmat Fees May Apply)
Greater than 98% by SDS-PAGE and HPLC analyses. Endotoxin level is less than 0.1 ng per μg of (1EU/μg) of TRAIL/Apo2L.
Description
Catalogue Number
GF092
Brand Family
Chemicon®
Trade Name
Chemicon
Description
TRAIL Protein, Recombinant human
Overview
Human TRAIL (TNF-Related Apoptosis Inducing Ligand), also called APO2 Ligand, is a cytotoxic protein which activates rapid apoptosis in tumor cells, but not in normal cells. TRAIL-induced apoptosis, is achieved through binding to two death-signaling receptors, DR4 and DR5. These receptors belong to the TNFR transmembrane family of proteins and contain a cytoplasmic "death domain" which activates the cells apoptotic machinery. GF092 is a 19.6 kDa protein comprising the full-length of the TNF-like extracellular domain of TRAIL.
Alternate Names
Apo2 Ligand
Product Information
Presentation
Sterile filtered, then lyophilized from 0.3 mg/mL in 10mM Sodium Phosphate + 110 mM NaCl.
Quality Level
MQ100
Applications
Application
Human TRAIL (TNF-Related Apoptosis Inducing Ligand), also called APO2 Ligand, is a cytotoxic protein which activates rapid apoptosis in tumor cells, but not in normal cells.
Key Applications
Cell Culture
Biological Information
Purity
Greater than 98% by SDS-PAGE and HPLC analyses. Endotoxin level is less than 0.1 ng per μg of (1EU/μg) of TRAIL/Apo2L.
Source
E.coli
Specific Activity
BIOLOGICAL ACTIVITY: Determined by its ability to induce apoptotic cell death in TRAIL-sensitive U343MG cells. The expected ED50 for this effect is 1.0-3.0 ng/mL.
Entrez Gene Number
NM_003810.2
Entrez Gene Summary
The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues. This protein binds to several members of TNF receptor superfamily including TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and possibly also to TNFRSF11B/OPG. The activity of this protein may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and TNFRSF11B/OPG that cannot induce apoptosis. The binding of this protein to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3.
Gene Symbol
TNFSF10
TL2
Apo-2L
CD253
TRAIL-PEN
APO2L
TRAIL
UniProt Number
P50591
UniProt Summary
FUNCTION: SwissProt: P50591 # Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis. COFACTOR: Binds 1 zinc ion per trimer. SIZE: 281 amino acids; 32509 Da SUBUNIT: Homotrimer. SUBCELLULAR LOCATION: Membrane; Single-pass type II membrane protein (Potential). TISSUE SPECIFICITY: Widespread; most predominant in spleen, lung and prostate. SIMILARITY: SwissProt: P50591 ## Belongs to the tumor necrosis factor family.
Stem Cell Type
Hematopoietic Stem Cells
Product Usage Statements
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
The lyophilized protein may be stored at room temperature for a few weeks, but is best stored at -20°C. After a quick spin, reconstitute in sterile water to a concentration of 0.5-1.0 mg/mL. This solution can be diluted into other buffered solutions and stored at 4ºC for 1 week or -20ºC for future use. Reconstituted GF092 should be stored in undiluted aliquots at -20°C for up to 6 months from date of receipt. Avoid repeated freeze/thaw cycles.
