Key Specifications Table
| Key Applications | Species | Source | Purity |
|---|---|---|---|
| PC | Human | Stable BHK (baby hamster kidney) cell transfectants | PEDF is purified via affinity purification from culture supernatants; Greater than 95% pure by SDS-PAGE analysis. Product may be rendered sterile by 0.22-micron filtration. |
| Description | |
|---|---|
| Catalogue Number | GF134 |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Pigment Epithelium Derived Factor Protein, Recombinant human |
| Overview | Pigment epithelium-derived factor (PEDF) was first identified as a 50 kDa secreted protein in conditioned medium from cultured fetal human retinal pigment epithelium (RPE) cells (Tombran-Tink et al., 1991). PEDF has been shown to have neurotrophic activity in the retina in vivo (Cayouette et al., 1999). PEDF is also a neurotrophic and neuroprotective factor in other systems, including cultured cerebellar granule cells, primary hippocampal neurons, spinal cord motor neurons and amphibian photoreceptors cultured in the absence of RPE cells (Karakousis, et al., 2001). The binding of PEDF to retinoblastoma and cerebellar granule cells may be receptor-mediated. Recently, PEDF was shown to be a potent inhibitor of angiogenesis in endothelial cell cultures and in rat cornea and retina in vivo (Dawson et al., 2001; Stellmach et al., 2001). |
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| Product Information | |
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| Presentation | Provided liquid in 20 mM sodium phosphate, pH 6.4, 0.2 M sodium chloride, and 1 mM DTT. Product has been sterile filtered to 0.22-micron. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | The Pigment epithelium-derived factor (PEDF) was first identified as a 50 kDa secreted protein in conditioned medium from cultured fetal human retinal pigment epithelium (RPE) cells (Tombran-Tink et al., 1991). |
| Key Applications |
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| Biological Information | |
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| Purity | PEDF is purified via affinity purification from culture supernatants; Greater than 95% pure by SDS-PAGE analysis. Product may be rendered sterile by 0.22-micron filtration. |
| Source | Stable BHK (baby hamster kidney) cell transfectants |
| Entrez Gene Number |
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| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P36955 # Neurotrophic protein; induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity. SIZE: 418 amino acids; 46342 Da SUBCELLULAR LOCATION: Secreted. Melanosome. Note=Enriched in stage I melanosomes. TISSUE SPECIFICITY: Retinal pigment epithelial cells and blood plasma. DEVELOPMENTAL STAGE: Expressed in quiescent cells. DOMAIN: SwissProt: P36955 The N-terminal (AA 44-121) exhibits neurite outgrowth- inducing activity. The C-terminal exposed loop (AA 382-418) is essential for serpin activity. PTM: The N-terminus is blocked. SIMILARITY: Belongs to the serpin family. |
| Product Usage Statements | |
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| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | Maintain frozen at -70°C in undiluted aliquots for up to 6 months from date of receipt. Avoid repeated freeze/thaw cycles. . |
| Packaging Information | |
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| Material Size | 10 µg |