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Millipore The Stem Cell Factor (Scf) Protein, Recombinant Mouse, Is A Hematopoietic Growth Factor That Exerts Its Activity At The Early Stages Of Hematopoiesis. - Mill (Additional S&H or Hazmat Fees May Apply)
Greater than 98% by SDS-PAGE. Endotoxin level is less than 0.1 ng per μg of SCF.
Description
Catalogue Number
GF141
Replaces
GF049
Brand Family
Chemicon®
Trade Name
Chemicon
Description
Stem Cell Factor Protein, Recombinant mouse
Overview
Stem Cell Factor (SCF) is a hematopoietic growth factor that exerts its activity at the early stages of hematopoiesis. SCF stimulates the proliferation of myeloid, erythroid, and lymphoid progenitors in bone marrow cultures and has been shown to act synergistically with colony stimulating factors. Murine SCF is an 18.3 kDa protein containing 164 amino acid residues.
Alternate Names
SCF
Mast Cell Growth Factor
MGF
c-kit Ligand
Product Information
Presentation
Lyophilized protein from 10 mm Sodium Phosphate, pH 6.5, containing no additives.
Quality Level
MQ100
Applications
Application
The Stem Cell Factor (SCF) Protein, Recombinant mouse, is a hematopoietic growth factor that exerts its activity at the early stages of hematopoiesis.
Application Notes
Concentration Range for most in vitro applications is 1.0 - 100 ng/mL. Optimal working dilutions must be determined by end user.
Biological Information
Purity
Greater than 98% by SDS-PAGE. Endotoxin level is less than 0.1 ng per μg of SCF.
Source
E. coli
Specific Activity
Murine SCF is biologically active when compared to standards. The ED(50), as determined by the dose-dependent stimulation of the proliferation of the human TF-1 cell line is <= 10 ng/mL, corresponding to a specific activity of >= 1x10e5 units/mg.
Gene Symbol
SCF
MGF
KITLG
UniProt Number
P21583
P20826
Stem Cell Type
Hematopoietic Stem Cells
Product Usage Statements
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
The lyophilized powder, though stable at room temperature, is best stored at -20°C for up to 12 months from date of receipt. After a quick spin, reconstitute with sterile water to a concentration of 0.1-1.0 mg/mL. This solution can be diluted into other buffered solutions and stored at 4°C for one week or at -20°C for future use. Reconstituted mouse SCF should be stored in working aliquots at -20°C for up to six months. Avoid repeated freeze/thaw cycles.
