Key Specifications Table
| Source | Purity |
|---|---|
| CHO Cells | Product is endotoxin free (< 0.1ng/μg of protein (<1EU/μg) and greater than 95% pure by SDS-PAGE gel and HPLC analyses |
| Description | |
|---|---|
| Catalogue Number | GF165 |
| Description | ANG-2 Protein, Human Recombinant |
| Alternate Names |
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| Background Information | Angiopoietin2 (Ang-2) is a secreted glycoprotein that plays a complex role in angiogenesis and inflammation. Ang-2 is widely expressed during development, but it is restricted postnatally to highly angiogenic tissues such as the placenta, ovaries, and uterus. It is particularly abundant in vascular endothelial cells (EC) where it is stored in intracellular Weibel Palade bodies. ANG-2 binds to the endothelial cell specific receptor Tie2, but, in contrast to ANG-1 does not induce tyrosine phosphorylation. Consequently, ANG-2 modulates ANG-1 activation of Tie2 and, depending on the physiological and biochemical environment, can act either as a n agonist or antagonist of Tie2 induced angiogenesis. Physiologically, ANG-1 and ANG-2 are associated with sprouting, tube formation, and structural integrity of newly formed blood vessels. Mature human ANG-2 is a secreted protein containing 480 amino acid residues. ANG-2 is composed of an α helix rich “coiled coil” N-terminal domain and fibrinogen like C-terminal domain. ANG-2 exists predominantly in the form of a disulfide-linked dimer. Recombinant human ANG-2 is a C-terminal histidine tagged glycoprotein which migrates with an apparent molecular mass of 60.0 – 70.0 kDa by SDS-PAGE under reducing conditions. Sequencing analysis shows an N-terminal sequence starting with residue 68 (D) of the ANG-2 precursor protein. |
| Product Information | |
|---|---|
| Presentation | Product is filtered through a 0.2 micron filter before lyophilization. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Angiopoietin2 (Ang-2) is a secreted glycoprotein that plays a complex role in angiogenesis & inflammation. |
| Biological Information | |
|---|---|
| Purity | Product is endotoxin free (< 0.1ng/μg of protein (<1EU/μg) and greater than 95% pure by SDS-PAGE gel and HPLC analyses |
| Source | CHO Cells |
| Cross Reactivity | None |
| UniProt Number |
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| Product Usage Statements | |
|---|---|
| Quality Assurance | Bioactivity assay: Determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC). Bioactivity assay: Determined by its ability to stimulate tubulogenesis in HUVEC cells using a concentration of 0.2μg/mL. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Store at -20°C for up to 6 months from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 20 µg |