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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | ELISA, IHC, NEUT | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB1021 |
| Replaces | 04-1114 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Tumor Necrosis Factor-α Antibody, clone 2C8 |
| Alternate Names |
|
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Liquid in PBS pH 7.4 containing 0.1% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect Tumor Necrosis Factor-α using this Anti-Tumor Necrosis Factor-α Antibody, clone 2C8 validated for use in ELISA, IH, NEUT. |
| Key Applications |
|
| Application Notes | Inhibition Assays – Inhibition of biological activity of recombinant and natural TNF-α. Detection of TNF-α in two-site immunoassay. Immunohistochemistry detected TNF-α. TNF-α Neutralization Assay 1. Plate 2 x 104 murine L929B cells into individual wells of 96-well (flat bottomed) microtiter plate. 2. Incubate overnight at 37°C in humidified 5% CO2 incubator. 3. The next day, prepare serial dilutions of anti-TNF (human) in medium containing 2 μg/mL of actinomycin D, for a final volume of 50 μL 4. Add 50 μL of TNF (10 ng/mL final concentration) to each antibody dilution and incubate for 1 hour at 37°C. 5. Add 100 μL of the antibody/antigen mixture to the pre-seeded L929B cells. 6. Incubate 24 hours at 37°C, 5% CO2. 7. Score wells for cytotoxicity. Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | Human recombinant tumor necrosis factor α. |
| Clone | 2C8 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | Human tumor necrosis factor-a (TNF-a) |
| Isotype | IgG1 |
| Species Reactivity |
|
| Species Reactivity Note | Human. No cross-reactivity observed with rat neuronal tissue. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. |
| Gene Symbol |
|
| Purification Method | Protein A Purfied |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P01375 # Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. SIZE: 233 amino acids; 25644 Da SUBUNIT: Homotrimer. SUBCELLULAR LOCATION: Cell membrane; Single-pass type II membrane protein. & Tumor necrosis factor, soluble form: Secreted. PTM: The soluble form derives from the membrane form by proteolytic processing. & The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1. DISEASE: SwissProt: P01375 # Cachexia accompanies a variety of diseases, including cancer and infection, and is characterized by general ill health and malnutrition. & Genetic variations in TNF are associated with susceptibility to psoriatic arthritis [MIM:607507]. Psoriasis is a chronic inflammatory dermatosis that affects approximately 2% of the population. It is characterized by red, scaly skin lesions that are usually found on the scalp, elbows, and knees, and may be associated with severe arthritis. Psoriatic arthritis has been defined as an inflammatory arthritis usually without any rheumatoid factor in serum (seronegative arthritis) associated with psoriasis. & Genetic variations in TNF are associated with susceptibility to hepatitis B virus infection (HBV infection) [MIM:610424]. Approximately one third of all cases of cirrhosis and half of all cases of hepatocellular carcinoma can be attributed to chronic HBV infection. HBV infection may result in subclinical or asymptomatic infection, acute self-limited hepatitis, or fulminant hepatitis requiring liver transplantation. SIMILARITY: SwissProt: P01375 ## Belongs to the tumor necrosis factor family. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at 2-8°C in undiluted aliquots for up to one year. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |