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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, R | ELISA, ICC, IHC, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB1059 |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Anti-Pigment Epithelium Derived Factor Antibody, clone 10F12.2 |
| Alternate Names |
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| Background Information | Pigment epithelium-derived factor (PEDF) was first identified as a 50 kDa secreted protein in conditioned medium from cultured fetal human retinal pigment epithelium (RPE) cells (Tombran-Tink, 1991). More recently, PEDF was shown to have neurotrophic activity in the retina in vivo (Cayouette, 1999). PEDF is also a neurotrophic and neuroprotective factor in other systems, including cultured cerebellar granule cells, primary hippocampal neurons, spinal cord motor neurons and amphibian photoreceptors cultured in the absence of RPE cells (Karakousis, 2001). The binding of PEDF to retinoblastoma and cerebellar granule cells may be receptor-mediated. Recently, PEDF was shown to be a potent inhibitor of angiogenesis in endothelial cell cultures and in rat cornea and retina in vivo (Dawson, 1999; Stellmach, 2001). |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified mouse monoclonal IgG2a in buffer containing 0.02 M PBS, pH 7.6, 0.25 M NaCl with 0.1% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Pigment Epithelium Derived Factor Antibody, clone 10F12.2 is an antibody against Pigment Epithelium Derived Factor for use in ELISA, IC, IH & WB. |
| Key Applications |
|
| Application Notes | Western Blot: Recognizes a protein with a molecular weight of ~46 kDa when tested against immunogen. Western blot positive control is available (Catalog number AG672) ELISA: A previous lot of this antibody was used on ELISA. Immunocytochemistry: A previous lot of this antibody was used on Immunocytochemistry. Immunohistochemistry: A previous lot of this antibody was used on frozen sections. Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | Recombinant human GST-PEDF fusion protein. The sequence corresponding to amino acids 196-347 of human PEDF tagged with GST on the N-terminal was expressed in E. Coli. GST-PEDF is about 46 kDa on SDS-PAGE. |
| Clone | 10F12.2 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | Recognizes Pigment-Epithelium Derived Factor (PEDF). |
| Isotype | IgG2a |
| Species Reactivity |
|
| Species Reactivity Note | Human. Expected to react with rat. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein A Purfied |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P36955 # Neurotrophic protein; induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity. SIZE: 418 amino acids; 46342 Da SUBCELLULAR LOCATION: Secreted. Melanosome. Note=Enriched in stage I melanosomes. TISSUE SPECIFICITY: Retinal pigment epithelial cells and blood plasma. DEVELOPMENTAL STAGE: Expressed in quiescent cells. DOMAIN: SwissProt: P36955 The N-terminal (AA 44-121) exhibits neurite outgrowth- inducing activity. The C-terminal exposed loop (AA 382-418) is essential for serpin activity. PTM: The N-terminus is blocked. SIMILARITY: Belongs to the serpin family |
| Molecular Weight | ~46 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by Western Blot on human placenta lysate. Western Blot Analysis: 1:1000 dilution of this lot detected pigment epithelium on 10 μg of human placenta lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 6 months at 2-8°C in undiluted aliquots from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |