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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | ELISA, RIA, WB, ICC, IH(P) | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB1061 |
| Replaces | AB1855 |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Anti-Bone Sialoprotein II Antibody, CT, clone ID1.2 |
| Alternate Names |
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| Background Information | Bone Sialoprotein II is one of the major noncollagenous proteins in the extracellular matrix of bone. It is a phosphorylated glycoprotein with an approximate molecular weight of 70 kDa. The protein has been found in osteoblasts and osteocytes. Diseases concerning bone turnover, pathological bone alterations, as well as the rapid increase of osteoporosis make it necessary to establish new markers. Bone Sialoprotein is discussed as a potential serum marker for monitoring bone remodeling. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified immunoglobulin from culture supernatant. Lyophilized from 50 mM TRIS, pH7.4. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Bone Sialoprotein II Antibody, C-terminus, clone ID1.2 is validated for use in ELISA, RIA, WB, IC, IH(P) for the detection of Bone Sialoprotein II. |
| Key Applications |
|
| Application Notes | Western blot: (1μg/mL) Immunohistochemistry on paraffin embedded tissue sections: (1μg/mL) Immunocytochemistry: (1:100) ELISA: (1μg/mL) RIA Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | Bone Sialoprotein isolated from human bones. |
| Epitope | C-terminus |
| Clone | ID1.2 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | Recombinant and native human bone sialoprotein II (BSPII). Does not react with osteopontin (BSPI) or osteonectin. |
| Isotype | IgG1 |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | The protein encoded by this gene is a major structural protein of the bone matrix. It constitutes approximately 12% of the noncollagenous proteins in human bone and is synthesized by skeletal-associated cell types, including hypertrophic chondrocytes, osteoblasts, osteocytes, and osteoclasts. The only extraskeletal site of its synthesis is the trophoblast. This protein binds to calcium and hydroxyapatite via its acidic amino acid clusters, and mediates cell attachment through an RGD sequence that recognizes the vitronectin receptor. |
| Gene Symbol |
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| Non-Reactive Species |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P21815 # Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Promotes Arg-Gly-Asp-dependent cell attachment. SIZE: 317 amino acids; 35018 Da SUBCELLULAR LOCATION: Secreted. PTM: N-glycosylated; glycans consist of sialated and core- fucosylated bi-, tri- and tetraantennary chains. & O-glycosylated at eight sites; mucin-type glycans contain Gal, GlcNAc, GalNAc and terminal NeuAc. MISCELLANEOUS: It is possible that the segments of clustered carboxyl groups mediate the strong binding to hydroxyapatite. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain lyophilized material at 2-8°C. Reconstitute with 100μL sterile distilled water and store at -20°C in undiluted aliquots up to 12 months. Avoid repeated freeze/thaw cycles. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |