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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | ELISA, IF, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB1072 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Erythropoietin Antibody, clone 1C8 |
| Alternate Names |
|
| Background Information | Erythropoietin (UniProt: P01588; also known as Epoetin, EPO) is encoded the EPO gene (Gene ID: 2056) in human. EPO is an acidic glycoprotein that is synthesized with a signal peptide (aa 1-27) that is subsequently cleaved off in the endoplasmic reticulum and the mature protein undergoes glycosylation in the Golgi prior to its release. EPO is mainly produced by peritubular fibroblasts in the renal cortex and to some extent by the liver in adult mammals. In fetal and neonatal mammals, it produced in the liver. EPO is the principle regulator of red blood cell production by stimulating the division and differentiation of bone marrow erythroid progenitor cells in response to anemia and hypoxia. It is induced by ischemic preconditioning by activation of (HIF)-1alpha. It is reported to contribute to angiogenesis in response to ischemia through upregulation of VEGF/VEGFR. It binds to EPO receptor that leads to receptor dimerization and JAK2 activation that results in activation of specific downstream effectors, including STAT1 and STAT3. Endogenous EPO consists of three N-linked polysaccharide groups and one O-linked group. Although its glycosylation slightly reduces its biological activity but is shown to be essential for ensuring its prolonged circulation in the plasma. The N-glycans in its structure protect it from the action of proteases and in the modulation of its receptor binding affinity. Mutations in EPO gene can cause microvascular complications in type II diabetic subjects and erythrocytosis. (Ref.: Bunn, HF (2013). Cold Spring Harb. Perspect. Med. 3(3); a011619). |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified mouse monoclonal IgG2b in buffer containing 20 mM sodium phosphate, 300 mM NaCl, and 0.02% sodium merthiolate. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect Erythropoietin using this Anti-Erythropoietin Antibody, clone 1C8 validated for use in ELISA, IF & WB. |
| Key Applications |
|
| Application Notes | ELISA: For the determination of Epo concentration in the range of 2-200 ng/mL. Use at 5 ug/mL to coat plate. Immunosorbent preparation Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | Recombinant human Epo. |
| Clone | 1C8 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | Reacts with human Erythropoietin (Epo). |
| Isotype | IgG2b |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene is a member of the EPO/TPO family and encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types. |
| Gene Symbol |
|
| Purification Method | Protein A Purfied |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P01588 # Erythropoietin is the principal hormone involved in the regulation of erythrocyte differentiation and the maintenance of a physiological level of circulating erythrocyte mass. SIZE: 193 amino acids; 21307 Da SUBCELLULAR LOCATION: Secreted. TISSUE SPECIFICITY: Produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals. SIMILARITY: SwissProt: P01588 ## Belongs to the EPO/TPO family. |
| Molecular Weight | 34 kDa (glycosylated) |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot on Mouse kidney lysates. Western Blot Analysis: 1:1000 dilution of this antibody detected glycosylated EPO on 10 µg of Mouse kidney lysates. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8ºC from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 250 µg |