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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | WB, IP, ICC, IHC | R | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB10750 |
| Description | Anti-LIMK1 Antibody, clone 40/00-1B11-1-1 |
| Alternate Names |
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| Background Information | LIM Kinase 1 (LIMK1) is a protein kinase that plays an important role in cellular functions like cell motility and growth cone actin dynamics. LIMK1 contains one PDZ domain and two LIM domains which mediate protein to protein inteactions. It is widely expressed in the adult and fetal nervous system with the highest levels being found in the cerebral cortex. Mutations in LIMK1 have been associated with cardiovascular and musculo-skeletal abnormalities. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-LIMK1 Antibody, clone 40/00-1B11-1-1 is validated for use in WB, IP, IC, IH for the detection of LIMK1. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant protein corresponding to a region of mouse LIMK1. |
| Epitope | Unknown |
| Clone | 40/00-1B11-1-1 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rat |
| Specificity | The antibody recognizes LIMK1. |
| Isotype | IgG2aκ |
| Species Reactivity |
|
| Species Reactivity Note | Human. Predicted to react with mouse based on 100% sequence homology. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is likely to be a component of an intracellular signaling pathway and may be involved in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome. |
| Gene Symbol |
|
| Purification Method | Protein G Purified |
| UniProt Number |
|
| UniProt Summary | FUNCTION: Protein kinase which regulates actin filament dynamics. Phosphorylates and inactivates the actin binding/depolymerizing factor cofilin, thereby stabilizing the actin cytoskeleton. CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein. SUBUNIT STURCTURE: Self-associates. Binds ROCK1 By similarity. The LIM domain interacts with the cytoplasmic domain of NRG1. Interacts with NISCH and SSH1. SUBCELLULAR LOCATION: Cytoplasm. TISSUE SPECIFICITY: Highest expression in the nervous system, particularly in the spinal cord and the cranial nerve and dorsal root ganglia. PTM: Autophosphorylated By similarity. Phosphorylated on serine and/or threonine residues by ROCK1. May be dephosphorylated and inactivated by SSH1. INVOLVEMENT IN DISEASE: Haploinsufficiency of LIMK1 may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in Williams-Beuren syndrome (WBS), a rare developmental disorder. It is a contiguous gene deletion syndrome involving genes from chromosome band 7q11.23. SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Contains 2 LIM zinc-binding domains. Contains 1 PDZ (DHR) domain. Contains 1 protein kinase domain. |
| Molecular Weight | ~ 73 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in PC3 cell lysate. Western Blot Analysis: 0.5 µg/ml of this antibody detected LIMK1 on 10 µg of PC3 cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |