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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB | M | Ascites | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB10765 |
| Description | Anti-Red Fluorescent Protein Antibody, clone 1DS-1A6 |
| Alternate Names |
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| Background Information | Since the development of Green fluorescent protein’s (GFP) use as a protein tag in chimeric proteins, fluorescent proteins have become widely used and useful biotechnological tools. The isolation of Red Fluorescent proteins have opened up a broader color spectrum for use in research. These proteins can be fused with a wide variety of proteins at either the N- or C-terminus, and have been expressed as fluorescent markers in most cell types. These markers can be used as noninvasive markers in both living organisms and cells. |
| Product Information | |
|---|---|
| Format | Ascites |
| Control |
|
| Presentation | Unpurified mouse monoclonal ascites with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Red Fluorescent Protein Antibody, clone 1DS-1A6 is validated for use in WB for the detection of Red Fluorescent Protein. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | Recombinant protein corresponding to sea anenome Red fluorescent protein. |
| Epitope | Unknown |
| Clone | 1DS-1A6 |
| Host | Mouse |
| Specificity | This antibody recognizes Red fluorescent protein. |
| Isotype | IgG1κ |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Gene Symbol |
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| Purification Method | Unpurified |
| UniProt Number |
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| UniProt Summary | FUNCTION: Thought to play a role in photoprotection of the corals resident symbiont microalgaes photosystems from photoinhibition caused by high light levels found near the surface of coral reefs. In deeper water, the fluorescence may be to convert blue light into longer wavelengths more suitable for use in photosynthesis by the microalgal symbionts. SUBUNIT STRUCTURE: Homotetramer. PTM: Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Xaa-N and Gly-N+2, oxidation of Tyr-N+1 to didehydrotyrosine, and formation of a double bond to the alpha-amino nitrogen of residue Xaa-N. Maturation of the chromophore requires nothing other than molecular oxygen. BIOTECHNOLOGICAL USE: Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. SEQUENCE SIMILARITIES: Belongs to the GFP family. BIOPHYSICOCHEMICAL PROPERTIES: Absorption: Exhibits a smaller absorbance peak at 494 nm. The broad fluorescence emission spectrum peaks at 583 nm. Abs(max)=558 nm |
| Molecular Weight | ~ 28 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in Cos cell lysate with overexpression of RFP. Western Blot Analysis: 1:1,000 dilution of this antibody detected Red fluorescent protein on 10 µg of Cos cell lysate with overexpression of RFP. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |