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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | FC, IHC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB1219 |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Anti-CD14 Antibody, clone 2D-15C |
| Overview | BIOCHEMISTRY: p I = 5.8 |
| Alternate Names |
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| Product Information | |
|---|---|
| Format | Purified |
| Presentation | The purified antibody is supplied in phosphate buffered saline, pH 7.4, containing 0.2% bovine serum albumin and 0.1% sodium azide. The characteristics of each lot are tested by electrophoresis and flow cytometry. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-CD14 Antibody, clone 2D-15C detects level of CD14 & has been published & validated for use in FC, IH. |
| Key Applications |
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| Application Notes | This antibody is an important general cell marker for mononuclear phagocytes in normal samples and a variety of disease states including a proportion of cases of myeloid leukemia. It is suitable for flow cytometry, and immunoperoxidase staining on frozen tissue sections. SUGGESTED USAGE DILUTION 1. Flow cytometry and indirect immunofluorescence 1:25 Dilute with isotonic buffer. Use 50 μl per 1 x 106 peripheral blood mononuclear cells (PBMC) in 100 μl buffer. 2. Indirect immunoperoxidase staining - the final dilution will depend on the assay conditions and detection system employed. However, a dilution of at least 1:25 will be applicable to most commonly used systems. |
| Biological Information | |
|---|---|
| Immunogen | Human peripheral blood mononuclear cells. |
| Clone | 2D-15C |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | This antibody belongs to CD14 (assigned by the Third International Workshop on Leucocyte Differentiation Antigens, Oxford, 1986; McMichael et al. Eds., 1986). and reacts with 50 to 55kD protein. It will detect blood monocytes, Kupffer cells, red pulp macrophages, dendritic cells and also "epithelioid" or giant cells within granulomatous tissue (Hancock et al., 1983), foam cells in renal interstitium (Nolasco et al., 1984) and placental dendritic cells and macrophages (Barnett et al., 1984; Brooks et al., 1983; Polli et al., 1984; Hopper et al., 1986). Monocytes react with this antibody in the early stages of differentiation, promonocytes and monoblasts do not. Activity is not lost on monocyte/macrophage activation (Hancock et al., 1983). Cell reactivity Reacts with peripheral blood monocytes and weakly with granulocytes. Negative to platelets, erythrocytes and lymphocytes. Sometimes positive for chronic myeloid leukemia (CML) and acute myeloid leukemia (AML). Negative to B-cell chronic lymphocytic leukemia (B-CLL), T-cell chronic lymphocytic leukemia (T-CLL) and common acute lymphoblastic leukemia (CALL). |
| Isotype | IgG1 |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | CD14 is a surface protein preferentially expressed on monocytes/macrophages. It binds lipopolysaccharide binding protein and recently has been shown to bind apoptotic cells. Alternative splicing results in multiple transcript variants encoding the same isoform. |
| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P08571 # Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules. SIZE: 375 amino acids; 40076 Da SUBUNIT: Belongs to the lipopolysaccharide (LPS) receptor, a multi-protein complex containing at least CD14, MD-2 and TLR4. SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor, GPI-anchor. TISSUE SPECIFICITY: Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages. SIMILARITY: SwissProt: P08571 ## Contains 11 LRR (leucine-rich) repeats. |
| Product Usage Statements | |
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| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Store at 2 to 8°C, for up to 6 months. For prolonged periods, store below -20°C in undiluted aliquots. AVOID REPEATED FREEZE/THAW CYCLES. WARNING: The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |