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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | FC, IHC, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB1222 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Tryptase Antibody, Mast Cell, clone G3 |
| Background Information | Tryptase is the most abundant secretory granule-derived serine proteinase contained in mast cells. Tryptase has recently been used as a marker for mast cell activation and is involved with allergic response. Tryptase may act as a mitogen for fibroblast lines. Elevated levels of serum tryptase occur in both anaphylactic and anaphylactoid reactions, but a negative test does not exclude anaphylaxis. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mm NaCl) with 0.05% sodium azide. (see product datasheet for specific buffer formulation) |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Tryptase Antibody, Mast Cell, clone G3 is validated for use in FC, IH, WB for the detection of Tryptase. |
| Key Applications |
|
| Application Notes | Flow Cytometry Analysis: A previous lot of this antibody has been used on fixed cells in FACS using a conjugated secondary antibody. (Sperr, W., et Al., 2001). Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Clone | G3 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | Recognizes Mast cell tryptase. Will show reactivity to basophils but to a lesser degree. |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | Tryptases comprise a family of trypsin-like serine proteases, the peptidase family S1. Tryptases are enzymatically active only as heparin-stabilized tetramers, and they are resistant to all known endogenous proteinase inhibitors. Several tryptase genes are clustered on chromosome 16p13.3. These genes are characterized by several distinct features. They have a highly conserved 3 UTR and contain tandem repeat sequences at the 5 flank and 3 UTR which are thought to play a role in regulation of the mRNA stability. These genes have an intron immediately upstream of the initiator Met codon, which separates the site of transcription initiation from protein coding sequence. This feature is characteristic of tryptases but is unusual in other genes. The alleles of this gene exhibit an unusual amount of sequence variation, such that the alleles were once thought to represent two separate genes, alpha and beta 1. Beta tryptases appear to be the main isoenzymes expressed in mast cells; whereas in basophils, alpha tryptases predominate. Tryptases have been implicated as mediators in the pathogenesis of asthma and other allergic and inflammatory disorders. |
| Gene Symbol |
|
| Purification Method | Protein g Purified |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P15157 # Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type.| Q15661 # Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. SIZE: 275 amino acids; 30773 Da SUBUNIT: Homotetramer. SUBCELLULAR LOCATION: Secreted. Note=Released from the secretory granules upon mast cell activation. DOMAIN:SwissProt: P15157 SIMILARITY: SwissProt: P15157 ## Belongs to the peptidase S1 family. Tryptase subfamily. & Contains 1 peptidase S1 domain. | Q15661 ## Belongs to the peptidase S1 family. Tryptase subfamily. & Contains 1 peptidase S1 domain. |
| Molecular Weight | 31 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by western blot on human fetal skin lysate. Western Blot Analysis: 0.5 µg/mL of this antibody detected tryptase in 10 µg of human fetal skin lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at 2–8°C for the duration listed on the product datasheet. (See product datasheet for storage conditions) |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |