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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IHC | M | Alkaline Phosphatase | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB1222A |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Tryptase Antibody, Mast Cell, clone G3, Alkaline Phosphatase |
| Product Information | |
|---|---|
| Format | Alkaline Phosphatase |
| Presentation | In 0.05M Tris + 0.1M NaCl + 0.001M MgCl2 with 0.1% NaN3, pH=8.0. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Tryptase Antibody, Mast Cell, clone G3, Alkaline Phosphatase is an antibody against Tryptase for use in WB, IH. |
| Key Applications |
|
| Application Notes | Immunoblotting & immunohistochemistry at 1:200-1:1,000. For optimal results, tissues or cytospin preparations should be fixed in Carnoys Fluid (60% ethanol, 30% chloroform, 10% glacial acetic acid). Not recommended for use on formaldehyde fixed tissue. May be used on thin sections prepared for ultrastructural analysis after fixation of tissue in a modified Karnovskys reagent [Lab. Invest. (1988). 58:682-691]. Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Clone | G3 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | Mast cell tryptase. Will show reactivity to basophils but to a lesser degree. |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | Tryptases comprise a family of trypsin-like serine proteases, the peptidase family S1. Tryptases are enzymatically active only as heparin-stabilized tetramers, and they are resistant to all known endogenous proteinase inhibitors. Several tryptase genes are clustered on chromosome 16p13.3. These genes are characterized by several distinct features. They have a highly conserved 3 UTR and contain tandem repeat sequences at the 5 flank and 3 UTR which are thought to play a role in regulation of the mRNA stability. These genes have an intron immediately upstream of the initiator Met codon, which separates the site of transcription initiation from protein coding sequence. This feature is characteristic of tryptases but is unusual in other genes. The alleles of this gene exhibit an unusual amount of sequence variation, such that the alleles were once thought to represent two separate genes, alpha and beta 1. Beta tryptases appear to be the main isoenzymes expressed in mast cells; whereas in basophils, alpha tryptases predominate. Tryptases have been implicated as mediators in the pathogenesis of asthma and other allergic and inflammatory disorders. |
| Gene Symbol |
|
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P15157 # Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type.| Q15661 # Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. SIZE: 275 amino acids; 30773 Da SUBUNIT: Homotetramer. SUBCELLULAR LOCATION: Secreted. Note=Released from the secretory granules upon mast cell activation. DOMAIN:SwissProt: P15157 SIMILARITY: SwissProt: P15157 ## Belongs to the peptidase S1 family. Tryptase subfamily. & Contains 1 peptidase S1 domain. | Q15661 ## Belongs to the peptidase S1 family. Tryptase subfamily. & Contains 1 peptidase S1 domain. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at +2-8°C in undiluted aliquots for up to 6 months. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |