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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, Rb | IHC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB13424 |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Anti-MMP-13 Antibody, clone VIIIA2 |
| Alternate Names |
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| Product Information | |
|---|---|
| Format | Purified |
| Control |
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| Presentation | Liquid in 10 mm PBS, pH 7.4, with 0.2% BSA and 15 mm sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-MMP-13 Antibody, clone VIIIA2 is validated for use in IH for the detection of MMP-13. |
| Key Applications |
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| Application Notes | Immunohistochemistry (frozen and formalin-fixed/paraffin*): 2-4 μg/mL for 60 minutes at room temperature. * Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mm citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at room temperature for 20 minutes. Immunoprecipation: use protein g (2 μg/mg protein lysate) Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | Human recombinant collagenase-3 protein. |
| Clone | VIIIA2 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | It recognizes proteins of ~60 kDa and ~48 kDa which are identified as pro (latent) and activate forms of matrix metalloproteinase-13 (MMP-13; also known as Collagenase-3). Shows no cross reactivity with the pro and active forms of other MMPs. Human collagenase-3 (MMP13) is a recently identified member of the matrix metalloproteinase (MMP) family that is expressed in breast carcinomas and in articular cartilage from arthritic patients. The MMP-13 gene has been isolated and characterized. This gene is composed of 10 exons and 9 introns and spans over 12.5 kb. The overall organization of the collagenase-3 gene is similar to that of other MMP genes clustered at chromosome 11q22, including fibroblast collagenase (MMP-1), matrilysin (MMP-7), and macrophage metalloelastase (MMP-12), but is more distantly related to genes coding for stromelysin-3 (MMP-11), gelatinase-A (MMP-2), and gelatinase-B (MMP-9), which map outside of this gene cluster. Cellular Localization: Cytoplasmic |
| Isotype | IgG1 |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The protein encoded by this gene cleaves type II collagen more efficiently than types I and III. It may be involved in articular cartilage turnover and cartilage pathophysiology associated with osteoarthritis. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. |
| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P45452 # Degrades collagen type I. Does not act on gelatin or casein. Could have a role in tumoral process. COFACTOR: Binds 4 calcium ions per subunit. & Binds 2 zinc ions per subunit. SIZE: 471 amino acids; 53820 Da TISSUE SPECIFICITY: Seems to be specific to breast carcinomas. DOMAIN: SwissProt: P45452 The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. DISEASE: SwissProt: P45452 # Defects in MMP13 are the cause of spondyloepimetaphyseal dysplasia type 2 (SEMD2) [MIM:602111]; also known as spondyloepimetaphyseal dysplasia type Missouri. SEMDs are a heterogeneous group of skeletal disorders characterized by defective growth and modeling of the spine and long bones. The SEMDs are distinguished from the spondylometaphyseal dysplasias and the spondyloepiphyseal dysplasias by the combined involvement of the epiphyses and metaphyses. The 3 disorders have malformations of the vertebrae in common. SIMILARITY: Belongs to the peptidase M10A family. & Contains 4 hemopexin-like domains. |
| Product Usage Statements | |
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| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at 2-8°C in undiluted aliquots for up to 12 months. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |