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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | FC, IP, IHC, FUNC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB1379 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-CD54 Antibody, extracellular, clone 84H10 |
| Alternate Names |
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| Background Information | This molecule is inducible to high levels by mitogenic lectins on lymphocytes and by IL-1beta or IFN gamma on other cell types such as fibroblasts and enthothelial cells. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Affinity purified immunoglobulin. Liquid in Phosphate buffered saline, pH 7.4, containing 0.09% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect CD54 using this Anti-CD54 Antibody, extracellular, clone 84H10 validated for use in FC, IP, IH. |
| Key Applications |
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| Applications Not Recommended |
|
| Application Notes | Flow Cytometry: Neat - 1:10. 10 μL of suggested dilution will label 10e6 cells. Immunohistochemistry on frozen tissue sections: 1:20-1:50. Does not work on paraffin embedded tissue sections. Immunoprecipitation: 5 μg/ 10e7 cells Adhesion Blocking; blocks ICAM-1 mediated adhesion to LFA-1. Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | K562 cell line |
| Epitope | extracellular |
| Clone | 84H10 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | Recognizes the extracellular D1 domain of ICAM-1. Blocks binding of LFA-1 and P. falciparum to ICAM-1. Reacts with the ICAM-1 antigen found in low levels on lymphocytes and strongly expressed on monocytes and granulocytes. Detects an antigen of 90 kDa. |
| Isotype | IgG1 |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | ICAM1 (CD54) is typically expressed on endothelial cells and cells of the immune system. ICAM1 binds to integrins of type CD11a / CD18, or CD11b / CD18. ICAM1 is also exploited by Rhinovirus as a receptor. |
| Gene Symbol |
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| Non-Reactive Species |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P05362 # ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). In case of rhinovirus infection acts as a cellular receptor for the virus. Also acts as receptor for some coxsackieviruses. SIZE: 532 amino acids; 57825 Da SUBUNIT: Interacts with human herpesvirus 8 MIR2 protein (Probable). SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. PTM: Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis. SIMILARITY: SwissProt: P05362 ## Belongs to the immunoglobulin superfamily. ICAM family. & Contains 5 Ig-like C2-type (immunoglobulin-like) domains. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Store at 2-8°C for up to 12 months from date of receipt. Alternatively, this antibody can be stored at -20ºC. Avoid repeated freeze-thaw cycles. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |