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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, Ca | EM, FC, ICC, IF, IHC, IP, Neutralization Assay | AHm | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB1398Z |
| Replaces | 04-1074 |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Anti-PECAM-1 Antibody, clone 2H8, Azide Free |
| Alternate Names |
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| Background Information | CD31, also known as platelet endothelial cell adhesion molecule 1 (PECAM1), is a type I integral membrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is constitutively expressed on the surface of endothelial cells, and concentrated at the junction between them. It is also weakly expressed on many peripheral lymphoid cells and platelets. CD31 has been used to measure angiogenesis in association with tumor recurrence. Other studies have also indicated that CD31 and CD34 can be used as markers for myeloid progenitor cells and recognize different subsets of myeloid leukemia infiltrates (granular sarcomas). |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
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| Presentation | Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, containing no preservatives. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-PECAM-1 Antibody, clone 2H8, Azide Free detects level of PECAM-1 & has been published & validated for use in Elecron Microscopy, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, and Neutralization Assay. |
| Key Applications |
|
| Application Notes | Immunofluorescence Analysis: Representative lots immunostained endothelial blood vessel cells in 4% paraformaldehyde-fixed frozen tumor sections from mice and dogs as a means for assessing lymph vessel density in tumor xenografts by fluorescent immunohistochemistry (Donat, U., et al. (2012). PLoS One. 7(9):e45942; Gentschev, I., et al. (2012). PLoS One.7(5):e37239). Immunofluorescence Analysis: A representative lot immunostained endothelial cells in formalin-fixed, paraffin-embedded murine peritoneum sections by fluorescent immunohistochemistry (Tanabe, K., et al. (2007). Kidney Int. 71(3):227-238). Immunocytochemistry Analysis: A representative lot immunostained 4% paraformaldehyde-fixed endothelial cells differentiated from murine ESCs in cultures (Purpura, K.A., et al. (2008). Stem Cells. 26(11):2832-2842). Immunocytochemistry Analysis: Clone 2H8 immunostained 3% paraformaldehyde-fixed, 0.5% NP-40-permeabilized COS-7 transfectants expressing murine, but not human, CD31 by fluorescent immunocytochemistry (Bogen, S.A., et al. (1992). Am. J. Pathol. 141(4):843-854). Neutralization Analysis: A representative lot, when injected intravenously in mice, blocked leukocyte emigration in response to thioglycollate injection (Bogen, S., et al. (1994). J. Exp. Med. 179(3):1059-1064). Neutralization Analysis: A representative lot blocked murine PECAM-mediated aggregation of mouse L cells (Xie, Y., and Muller, W.A. (1993). Proc. Natl. Acad. Sci. U. S. A. 190(12):5569-5573). Flow Cytometry Analysis: A representative lot detected the expression of exogenously transfected murine CD31 on the surface of mouse L cells (Xie, Y., and Muller, W.A. (1993). Proc. Natl. Acad. Sci. U. S. A. 190(12):5569-5573). Flow Cytometry Analysis: Clone 2H8 detected CD31 immunoreactivity on the surface of various murine B- and T-cell lines (Bogen, S.A., et al. (1992). Am. J. Pathol. 141(4):843-854). Immunoprecipitation Analysis: A representative lot immunoprecipitated exogenously expressed murine, but not human, CD31 from COS-7 transfectants (Xie, Y., and Muller, W.A. (1993). Proc. Natl. Acad. Sci. U. S. A. 190(12):5569-5573). Immunoprecipitation Analysis: Clone 2H8 immunoprecipitated CD31 from murine CTLL T lymphocytes and Sp2/0 B lymphocytes (Bogen, S.A., et al. (1992). Am. J. Pathol. 141(4):843-854). Electron Microscopy Analysis: Clone 2H8 was biotinylated and immunostained lymphocytes in 0.01% glutaraldehyde-/2% formaldehyde-fxed frozen reactive lymph node tissues from KLH-immunized mice (Bogen, S.A., et al. (1992). Am. J. Pathol. 141(4):843-854). Immunohistochemistry Analysis: Clone 2H8 immunostained lymphocytes adherent to the sinusoidal and postcapillary venular endothelium of frozen reactive lymph node tissues from KLH-immunized mice (Bogen, S.A., et al. (1992). Am. J. Pathol. 141(4):843-854). |
| Biological Information | |
|---|---|
| Immunogen | D10.G4.1 murine T cells (Bogen, S.A., et al. (1992). Am. J. Pathol. 141(4):843 -854). |
| Epitope | Extracellular domain. |
| Clone | 2H8 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Armenian Hamster |
| Specificity | React s with the 130-140 kDa protein, PECAM-1 found on mouse monocytes, platelets, granulocytes and endothelial cells. 2H8 effectively inhibits transmigration of activated polymophonuclear cells and monocytes across the endothelium. In a mouse model for acute peritonis - 2H8 blocks acute inflammation. |
| Isotype | IgG |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein A Purfied |
| UniProt Number |
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| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |