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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Ch, H, Mk, R, Rb | WB, IHC, IH(P) | M | Ascites | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB1522 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Actin Antibody, smooth muscle γ & α actin, clone CGA7 |
| Product Information | |
|---|---|
| Format | Ascites |
| Presentation | Ascites. Liquid |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect Actin using this Anti-Actin Antibody, smooth muscle γ & α actin, clone CGA7 validated for use in WB, IH, IH(P). |
| Key Applications |
|
| Application Notes | Western Blot: 1:1000 (approximately 43 kDa) Immunohistochemistry (paraffin, formalin, deparaffinized methacarn-fixed tissue: 1:10,000 or alcohol fixation) Optimal dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | Chicken gizzard actin |
| Epitope | smooth muscle gamma & alpha actin |
| Clone | CGA7 |
| Host | Mouse |
| Specificity | Antibody recognizes actin isotypes alpha and gamma of smooth muscle cells, but not skeletal or cardiac muscle cells. Reactive with myoepithelium, but nonreactive with other mesenchymal and all epithelial cells. The antibody labels eiomyosarcomas and leiomyomas but does not react with other neoplasms such as carcinomas or lymphoma. |
| Isotype | IgG2a |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | Actins are highly conserved proteins that are involved in various types of cell motility, and maintenance of the cytoskeleton. In vertebrates, three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. Actin, gamma 2, encoded by this gene, is a smooth muscle actin found in enteric tissues. |
| Gene Symbol |
|
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P63267 # Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. SIZE: 376 amino acids; 41877 Da SUBUNIT: Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton. SIMILARITY: SwissProt: P63267 ## Belongs to the actin family. MISCELLANEOUS: In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain frozen at -20°C for up to 12 months in undiluted aliquots. Avoid repeated freeze/thaw cycles. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |