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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| B, H, M, Po, R | ICC, IHC, WB | M | Culture Supernatant | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB1527 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Peripherin Antibody, clone 8G2 |
| Background Information | Peripherin is a ~57kDa intermediate filament subunit found initially in sensory neurons of the peripheral nervous systems. Subsequently it was found in some sensory and other neurons of the central nervous system and also in PC12 cells. Peripherin is also expressed in certain neuroendocrine tumors and in the insulin producing cells of the pancreas. Peripherin belongs to the Class III family of intermediate filament subunits which includes vimentin, glial fibrillary acidic protein (GFAP) and desmin. Antibodies to peripherin can be used in identifying, classifying, and studying neurons throughout the nervous system. Peripherin is also a good diagnostic marker for ballooned axons seen in Lou Gehrigs disease (Amyotrophic lateral sclerosis) and some neuronally derived tumors. Autoantibodies to peripherin are frequently seen in the sera of patients with diabetes. Peripherin is not related to peripherin/RDS, a protein of the photoreceptor outer membrane mutations of which are causative of certain forms of slow retinal degeneration. |
| Product Information | |
|---|---|
| Format | Culture Supernatant |
| Control |
|
| Presentation | UnPurified mouse culture supernatant containing no preservatives. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Peripherin Antibody, clone 8G2 is an antibody against Peripherin for use in IC, IH & WB. |
| Key Applications |
|
| Applications Not Recommended |
|
| Application Notes | Immunocytochemistry: 4% PFA fixed cells (5 minutes) or acetone is recommended. Immunohistochemisty: light PFA fixation is necessary as 8G2 is sensitive to heavy formalin fixation. Antigen recovery is enhanced with 0.1% triton in the block only. 1:25-1:200 Immunoblotting: The antibody is clean and specific for the expected 57kDa band on Western blots. Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | Electrophoretically pure trp-E-peripherin fusion protein [Dev. Brain Res. (1990). 57:235-248] containing all but the 4 N-terminal amino acids of rat peripherin. Fusion protein purified from bacterial inclusion bodies by DEAE-cellulose chromatography in 6M urea followed by preparative SDS-PAGE |
| Clone | 8G2 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | Recognizes Peripherin. Strong staining on rat, mouse, human, pig and cow peripherin. Does not work on chicken or other more distantly related species, which may lack peripherin. |
| Isotype | IgG |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene encodes a cytoskeletal protein found in neurons of the peripheral nervous system. The encoded protein is a type III intermediate filament protein with homology to other cytoskeletal proteins such as desmin, and is a different protein that the peripherin found in photoreceptors. Mutations in this gene have been associated with susceptibility to amyotrophic lateral sclerosis. |
| Gene Symbol |
|
| Purification Method | Unpurified |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P41219 # Class-III neuronal intermediate filament protein. SIZE: 470 amino acids; 53651 Da SIMILARITY: SwissProt: P41219 ## Belongs to the intermediate filament family. |
| Molecular Weight | 57kDa |
| Product Usage Statements | |
|---|---|
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain for 12 months at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| Packaging Information | |
|---|---|
| Material Size | 300 µL |