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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R, Gp | IH(P), ICC, FC, ELISA | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB1913-C |
| Description | Anti-Procollagen Type I Antibody, CT, clone PCIDG10 (Ascites Free) |
| Alternate Names |
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| Background Information | Collagen alpha-1(I) chain (UniProt P02452; also known as Alpha-1 type I collagen) is encoded by the COL1A1 gene (Gene ID 1277) in human. Collagen is the major component of the extracellular matrix (ECM) and forms the fibrils of tendons, ligaments, and bones. Type I collagen consists of two alpha I chains and one alpha 2 chain. Alpha-1 type I collagen is initially produced as a 1464-amino acid prepro-form with a signal peptide sequence (a.a. 1-22) and two propeptide sequences (a.a. 23-161 and a.a. 1219 –1464), the removal of which yields the mature alpha-1(I) chain. The mature alpha-1(I) chain is composed mostly of a large triple-helical region (a.a. 179-1192) sandwiched between two nonhelical segments known as the N-terminal telopeptide (a.a. 162-178; numbering based on the prepro-form) and the C-terminal telopeptide (a.a. 1193-1218; numbering based on the prepro-form). Collagen can be extracted from tissue via either enzymatic or non-enzymatic means. Collagen extracted using the proteolytic enzyme pepsin corresponds to the large triple-helical region, referred to as atelocollagen because both the N- and C-terminal telopeptides have been cleaved off by pepsin. On the other hand, collagen preparations obtained with non-enzymatic means (e.g. by acid extraction) have the intact telopeptides at both ends. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl without preservatives. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Procollagen Type I Antibody, CT, clone PCIDG10 (Ascites Free) is validated for use in Immunohistochemistry (Paraffin), Immunocytochemistry, Flow Cytometry and ELISA for the detection of Procollagen Type I. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Procollagen Type I in mouse skin, rat skin, and rat skeletal muscle tissue. Immunocytochemistry Analysis: A representative lot detected type I procollagen immunoreactivity in human semitendinosus and gracilis tendon fibroblasts from patients undergoing reconstruction surgery after anterior cruciate ligament (ACL) rupture by fluorescent immunocytochemistry (Bayer, M.L., et al. (2012). Mech Ageing Dev. 133(5):246-254). Flow Cytometry Analysis: A representative lot detected PICP+/CD45+ fibrocytes in lung cells from bleomycin-treated mice (Yeager, M.E., et al. (2012). Eur Respir J. 39(1):104-111). Flow Cytometry Analysis: A representative lot detected higher numbers and percentages of circulating PICP+/CD45+ fibrocytes in peripheral blood samples from children/yound adults with pulmonary hypertension (PH) than in samples from healthy individuals (Reese, C., et al. (2014). Front Pharmacol. 5:141). Immunohistochemistry Analysis: A representative lot detected cytoplasmic type I procollagen immunoreactivity in stromal cells from the lysed functionalis of frozen human menstrual endometria sections (Gaide Chevronnay, H.P., et al. (2009). Endocrinology. 150(11):5094-5105). ELISA Analysis: A representative lot detected different age-dependency of procollagen type I C-terminal propeptide (PICP) immunoreactivity in the cruciate ligaments of osteoarthritis-/OA-prone Dunkin-Hartley (DH) guinea pigs and in age-matched Bristol strain 2 (BS2) control guinea pigs (Quasnichka, H.L., et al. (2005). Arthritis Rheum. 52(10):3100-3109). |
| Biological Information | |
|---|---|
| Immunogen | Human pro-collagen I. |
| Epitope | C-terminal propeptide region |
| Clone | PCIDG10 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Labels carboxy-terminal pro-peptide of collagen type I. Does not stain mature collagen fibers in tissue, but rather is localized intracellularly in cells producing pro-collagen I. |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Species Reactivity Note | Human, Mouse, Rat, Guinea Pig. Predicted to react with Bovine based on 100% sequence homology. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G Purified |
| UniProt Number |
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| Molecular Weight | 140 kDa calculated |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunohistochemistry in human bone tissue. Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected Procollagen Type I in human bone tissue. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |