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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, Mk, Rb | ELISA, CULT, IF, IHC, IP, WB | M | Ascites | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB1922 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Laminin α2 Antibody, clone 5H2 |
| Alternate Names |
|
| Background Information | Elements of the extracellular matrix such as laminins, a family of heterotrimeric extracellular glycoproteins, affect tissue development and integrity in organs including the kidney, lung, skin, and nervous system. Laminins function as heterotrimeric complexes of alpha, beta, and gamma chains, with each chain type representing a different subfamily of proteins. For example, the alpha subfamily of laminin chains is a major component of basement membranes. Two transcript variants encoding different isoforms have been found for this gene, but the full-length nature of one of them has not been determined. At least 15 distinct laminin trimers, containing various combinations of 5 alpha, 4 beta, and 3 gamma subunits have been found in mammals. Both laminin alpha 5 and alpha 1 are prevalent in kidney. Mice that are homozygous for a null mutation in the alpha 5 laminin gene are dead by embrionic day 14 - 19 with multiple developmental abnormalities. The kidney phenotypes include avascular glomeruli, impaired branching morphogenesis, and renal agenesis. |
| Product Information | |
|---|---|
| Format | Ascites |
| Control |
|
| Presentation | Unpurified ascites in buffer containing no preservatives. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect Laminin α2 using this Anti-Laminin α2 Antibody, clone 5H2 validated for use in ELISA, CULT, IF, IH, IP & WB. |
| Key Applications |
|
| Application Notes | ELISA: 50% maximal binding to human merosin at 1:50,000 dilution from a previous lot. Immunohistochemistry: 1:5,000 dilution from a previous lot was used for staining of 8 µM acetone-fixed cryostat muscle sections, prior to detection with a peroxidase-conjugated secondary antibody. Immunofluorescence: A previous lot of this antibody was used in immunofluorescent. Affinity chromatography: A previous lot of this antibody was used in IAP. Immunoprecipitation: A previous lot of this antibody was used in IP. Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | Purified human merosin |
| Clone | 5H2 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | Reacts with the 80 kDa fragment of the M-chain of human merosin. |
| Isotype | IgG1 |
| Species Reactivity |
|
| Species Reactivity Note | Cross reacts with monkey and rabbit merosin. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | Laminin, an extracellular protein, is a major component of the basement membrane. It is thought to mediate the attachment, migration, and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. It is composed of three subunits, alpha, beta, and gamma, which are bound to each other by disulfide bonds into a cross-shaped molecule. This gene encodes the alpha 2 chain, which constitutes one of the subunits of laminin 2 (merosin) and laminin 4 (s-merosin). Mutations in this gene have been identified as the cause of congenital merosin-deficient muscular dystrophy. Two transcript variants encoding different proteins have been found for this gene. |
| Gene Symbol |
|
| Purification Method | Unpurified |
| UniProt Number |
|
| UniProt Summary | |
| Molecular Weight | 80 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by Western Blot on Human Placenta lysate. Western Blot Analysis: 1:1000 dilution of this lot detected Laminin α2 (merosin) on 10 μg of Human Placenta lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage the IgG1 and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |