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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, Mk, Rb | ELISA, FC, ICC, IHC, IH(P), IP, FUNC, CULT | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB1951 |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Anti-Integrin β1 Antibody, clone P4G11 |
| Alternate Names |
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| Product Information | |
|---|---|
| Format | Purified |
| Control |
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| Presentation | Protein A Purified immunoglobulin in 0.02M PBS, 0.25M NaCl, pH 7.6, with 0.1% NaN3. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Integrin β1 Antibody, clone P4G11 is an antibody against Integrin β1 for use in ELISA, FC, IC, IH, IH(P), IP, FUNC, CULT. |
| Key Applications |
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| Application Notes | Immunohistochemistry: for use on acetone or mild paraformaldehyde fixed tissue (2-2.5% PFA, short time) frozen and 4% PFA paraffin embedded {Qin et al 2003 1:500, citrate HEIR treated} Ca++ required for binding. Ca++ must be used in blocking, washing, and antibody incubation buffers. Traditional formalin fixation is not recommended. Immunocytochemistry: 2 ug/mL (for use on numerous cell lines as long as Ca2+ is present) Immunoprecipitation: 5 μg/400 μL lysate (0.5% triton PBS plus Ca++) FACS: 2-5μg per 10e6 cells, in the presence of Ca++. Stimulation of cells: 2-10 μg/ml per 10e6 cells. Binding buffer must contain 1mM Ca++. ELISA Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | Human IL-1beta activated endothelial cells |
| Clone | P4G11 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | Reacts with calcium-dependent epitopes on the integrin β1 subunit. When bound to β1, P4G11, activates β1 dependent binding to extra cellular matrix substrates. |
| Isotype | IgG1 |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene. |
| Gene Symbol |
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| Purification Method | Protein A Purfied |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P05556 # Integrins alpha-1/beta-1, alpha-2/beta-1, alpha-10/beta- 1 and alpha-11/beta-1 are receptors for collagen. Integrins alpha- 1/beta-1 and alpha-2/beta-2 recognize the proline-hydroxylated sequence G-F-P-G-E-R in collagen. Integrins alpha-2/beta-1, alpha- 3/beta-1, alpha-4/beta-1, alpha-5/beta-1, alpha-8/beta-1, alpha- 10/beta-1, alpha-11/beta-1 and alpha-V/beta-1 are receptors for fibronectin. Alpha-4/beta-1 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. Integrin alpha-5/beta-1 is a receptor for fibrinogen. Integrin alpha-1/beta-1, alpha-2/beta-1, alpha-6/beta-1 and alpha-7/beta-1 are receptors for lamimin. Integrin alpha-4/beta-1 is a receptor for VCAM1. It recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-9/beta-1 is a receptor for VCAM1, cytotactin and osteopontin. It recognizes the sequence A-E-I-D-G-I-E-L in cytotactin. Integrin alpha-3/beta-1 is a receptor for epiligrin, thrombospondin and CSPG4. Alpha-3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. Integrin alpha-V/beta-1 is a receptor for vitronectin. Beta-1 integrins recognize the sequence R-G-D in a wide array of ligands. Isoform beta-1B interferes with isoform beta-1A resulting in a dominant negative effect on cell adhesion and migration (in vitro). In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposis sarcoma lesions. SIZE: 798 amino acids; 88465 Da SUBUNIT: Heterodimer of an alpha and a beta subunit. Beta-1 associates with either alpha-1, alpha-2, alpha-3, alpha-4, alpha- 5, alpha-6, alpha-7, alpha-8, alpha-9, alpha-10, alpha-11 or alpha-V. Interacts with FLNA. Binds LGALS3BP and ITGB1BP3, when associated with alpha-7, but not with alpha-5 (By similarity). Interacts with FLNB and RANBP9. Isoform Beta-1D interacts with ACE2. Isoform Beta-1A interacts with the C-terminal region of FLNC. Interacts with HIV-1 Tat. SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. Melanosome. Note=Isoform beta-1B does not localize to focal adhesions. Highly enriched in stage I melanosomes. TISSUE SPECIFICITY: Isoform beta-1A is widely expressed, other isoforms are generally coexpressed with a more restricted distribution. Isoform beta-1B is expressed in skin, liver, skeletal muscle, cardiac muscle, placenta, umbelical vein endothelial cells, neuroblastoma cells, lymphoma cells, hepatoma cells and astrocytoma cells. Isoform beta-1C and isoform beta-1C-2 are expressed in muscle, kidney, liver, placenta, cervical epithelium, umbilical vein endothelial cells, fibroblast cells, embryonal kidney cells, platelets and several blood cell lines. Isoform beta-C-2, rather than isoform beta-1C, is selectively expressed in primary T-cells. Isoform beta-1C is expressed in nonproliferating and differentiated prostate gland epithelial cells. Isoform beta-1D is expressed specifically in striated muscle (skeletal and cardiac muscle). SIMILARITY: SwissProt: P05556 ## Belongs to the integrin beta chain family. & Contains 1 VWFA domain. |
| Product Usage Statements | |
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| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |