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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, Ch, Ht | FC | M | Phycoerythrin | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB1961H-I |
| Description | Anti-Integrin αVβ5 Antibody, clone P1F6, Phycoerythrin conjugate |
| Alternate Names |
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| Background Information | Integrin alpha-V (UniProt P06756; CD51, Integrin alpha V, Vitronectin receptor subunit alpha) and Integrin beta-5 (UniProt P18084) are encoded by the ITGAV (MSK8, VNRA, VTNR; Gene ID 3685) and the ITGB5 (Gene ID 3693) gene, respectively, in human. The integrin family of cell adhesion receptors consists of at least 16 membrane-associated heterodimers, composed of an alpha and a beta subunit associated in a non-covalent manner. The structure and functional diversity of the integrin family are based upon the pairing abilities of the individual alpha and beta subunits. Key to these molecular interactions between the integrin receptors and their respective ligands is the recognition of the Arg-Gly-Asp (RGD) sequence present in the extracellular matrix components, such as fibronectin, vitronectin, collagen, fibrinogen, and von Willebrand factor. The involvement of integrins in vascular proliferation, adhesion, and wound repair has been well documented. Integrin alphaVbeta5 is the primary mechanotransducer that mediates lung myofibroblast contraction-induced latent TGF-beta1 activation. Increased alphaVbeta5 expression is reported to be associated with myofibroblast differentiation. Integrin alphaVbeta5 is shown to interact with Thy-1 in an RLD-dependent manner. Thy-1 expression prevents fibroblast contraction-induced latent TGF-beta1 activation and lung myofibroblast differentiation. |
| Product Information | |
|---|---|
| Format | Phycoerythrin |
| Presentation | Purified mouse monoclonal IgG1 antibody in PBS with 15 mg/mL BSA and 0.1 % sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Integrin αVβ5 Antibody, clone P1F6, Phycoerythrin conjugate is an antibody against Integrin αVβ5 for use in Flow Cytometry. |
| Key Applications |
|
| Application Notes | The unconjugated antibody (Cat. No. MAB1961 & MAB1961Z) is shown to be suitable also for Flow cytometry, Functional blocking, Immunocytochemistry, Immunohistochemistry, and Immunoprecipitation applications. |
| Biological Information | |
|---|---|
| Immunogen | Human UCLA P3 lung carcinoma cells (Weinacker, A., et Al. (1994). J. Biol. Chem. 269(9):6940-6948). |
| Epitope | Extracellular region. |
| Clone | P1F6 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone P1F6 recognizes αVβ5 without cross-reactivity toward αVβ3 (Weinacker, A., et Al. (1994). J. Biol. Chem. 269(9):6940-6948). |
| Isotype | IgG1 |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Protein A Purfied |
| UniProt Number |
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| Molecular Weight | 112.7 kDa (alpha-V) and 86.34 kDa (beta-5) calculated. ~150 kDa (alpha-V) and 95 kDa (beta-5) reported (Weinacker, A., et Al. (1994). J. Biol. Chem. 269(9):6940-6948) due to glycosylation. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Flow Cytometry in HT1080 cells. Flow Cytometry Analysis: 1.0 µg of this antibody detected Integrin αVβ5 in HT1080 cells. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |