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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | ELISA, FC, ICC, IHC, IP, FUNC, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB1973 |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Anti-Integrin α1 Antibody, clone FB12 |
| Alternate Names |
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| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified immunoglobulin in 0.02M PB, pH 7.6, 0.25M NaCl containing 0.1% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Integrin α1 Antibody, clone FB12 is validated for use in ELISA, FC, IC, IH, IP, FUNC, WB for the detection of Integrin α1. |
| Key Applications |
|
| Application Notes | Immunohistochemistry: 2 μg/mL, with paraformaldehyde fixation. Immunohistochemistry: Frozen sections (7 µm) of placental tissue fixed with acetone for 1 hr. Antibody used at 1:10 dilution (Sato et al., 2003). Immunocytochemistry: iolated EVT cells firxed with 0.5% PFA @ 4°C for 15 minutes followed by acetone at -20°C for 2 minutes. Antibody was used at 1:10 dilution (Sato et al., 2003). Immunoblotting: 2 μg/mL Immunoprecipitation: 1 μg/mL FACS analysis: 2μg/mL (Sato et al., 2005). ELISA: 0.5 μg/mL Inhibition of activated human lymphocyte binding to laminin, collagen IV and fibronectin (Fabbri et al., 1996): 1-5 μg/mL Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Clone | FB12 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | Reacts with the I domain (Val151 - Ala364) of human alpha-1 integrin (CD49a)1. Precipitates proteins of Mr 180 kDa, pI 5.9-6.0 (alpha chain) and Mr 115 kDa, pI 4.5-5.0 (beta chain) from human lymphocyte lysates (Zocchi et al., 1991). |
| Isotype | IgG1 |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | This gene encodes the alpha 1 subunit of integrin receptors. This protein heterodimerizes with the beta 1 subunit to form a cell-surface receptor for collagen and laminin. The heterodimeric receptor is involved in cell-cell adhesion and may play a role in inflammation and fibrosis. The alpha 1 subunit contains an inserted (I) von Willebrand factor type I domain which is thought to be involved in collagen binding. |
| Gene Symbol |
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| Purification Method | Protein A Purfied |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P56199 # Integrin alpha-1/beta-1 is a receptor for laminin and collagen. It recognizes the proline-hydroxylated sequence G-F-P-G- E-R in collagen. SIZE: 1179 amino acids; 130848 Da SUBUNIT: Heterodimer of an alpha and a beta subunit. Alpha-1 associates with beta-1. SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. DOMAIN: SwissProt: P56199 The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage. SIMILARITY: Belongs to the integrin alpha chain family. & Contains 7 FG-GAP repeats. & Contains 1 VWFA domain. |
| Molecular Weight | 130 kDa |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |