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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| B, Ca, Ch, H, Po, Rb, Sh | ELISA, RIA, WB, IHC | M | Ascites | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB2015 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Cartilage Proteoglycan Antibody, adult, clone EFG-4 |
| Product Information | |
|---|---|
| Format | Ascites |
| Presentation | Ascites fluid |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Cartilage Proteoglycan Antibody, adult, clone EFG-4 detects level of Cartilage Proteoglycan & has been published & validated for use in ELISA, RIA, WB, IH. |
| Key Applications |
|
| Application Notes | Western blot: 1:1,00 - 1:200 Immunohistochemistry: 1:50 - 250 ELISA: 1:250 - 1: 2500 RIA: 1:20,000 - 1:40,000 |
| Biological Information | |
|---|---|
| Immunogen | Human adult cartilage proteoglycan. |
| Epitope | adult |
| Clone | EFG-4 |
| Host | Mouse |
| Specificity | Recognizes the short peptides substituted with keratan sulfate side chains and within the core protein of proteoglycans in articular cartilages, chicken limb bud and cornea. Does not cross-react with human fetal cartilage proteoglycans. |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | This gene is a member of the aggrecan/versican proteoglycan family. The encoded protein is an integral part of the extracellular matrix in cartilagenous tissue and it withstands compression in cartilage. Mutations in this gene may be involved in skeletal dysplasia and spinal degeneration. Multiple alternatively spliced transcript variants that encode different protein isoforms have been observed in this gene. |
| Gene Symbol |
|
| Non-Reactive Species |
|
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P16112 # This proteoglycan is a major component of extracellular matrix of cartilagenous tissues. A major function of this protein is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region. SIZE: 2415 amino acids; 250193 Da SUBUNIT: Interacts with FBLN1 (By similarity). SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix (By similarity). TISSUE SPECIFICITY: Restricted to cartilages. DEVELOPMENTAL STAGE: Expression was detected in chondrocytes throughout the developing skeleton. DOMAIN: SwissProt: P16112 Two globular domains, G1 and G2, comprise the N-terminus of the proteoglycan, while another globular region, G3, makes up the C-terminus. G1 contains Link domains and thus consists of three disulfide-bonded loop structures designated as the A, B, B motifs. G2 is similar to G1. The keratan sulfate (KS) and the chondroitin sulfate (CS) attachment domains lie between G2 and G3. PTM: Contains mostly chondroitin sulfate, but also keratan sulfate chains, N-linked and O-linked oligosaccharides. The release of aggrecan fragments from articular cartilage into the synovial fluid at all stages of human osteoarthritis is the result of cleavage by aggrecanase. DISEASE: SwissProt: P16112 # Defects in AGC1 are the cause of spondyloepiphyseal dysplasia type Kimberley (SEDK) [MIM:608361]. Spondyloepiphyseal dysplasias are a heterogeneous group of congenital chondrodysplasias that specifically affect epiphyses and vertebrae. The autosomal dominant SEDK is associated with premature degenerative arthropathy. SIMILARITY: Belongs to the aggrecan/versican proteoglycan family. & Contains 1 C-type lectin domain. & Contains 1 EGF-like domain. & Contains 1 Ig-like V-type (immunoglobulin-like) domain. & Contains 4 Link domains. & Contains 1 Sushi (CCP/SCR) domain. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at -20°C in aliquots for up to 12 months. Avoid repeated freeze/thaw cycles. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |