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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| B, Ca, H, M, Po, Sh | ELISA, RIA, WB | M | Ascites | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB2022 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Keratan Sulfate Antibody, clone EFG-11 |
| Product Information | |
|---|---|
| Format | Ascites |
| Presentation | Ascites liquid, no preservative. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Keratan Sulfate Antibody, clone EFG-11 is an antibody against Keratan Sulfate for use in ELISA, RIA & WB. |
| Key Applications |
|
| Application Notes | Western blot at 1:1000 - 1:4000 ELISA at 1:5,000-1:20,000 RIA at 1:30,000-1:500,000 May be used in a competitive inhibition assay to measure keratan sulfate levels. |
| Biological Information | |
|---|---|
| Immunogen | Human articular cartilage proteoglycan digested with chondroitinase ABC. |
| Clone | EFG-11 |
| Host | Mouse |
| Specificity | Recognizes 2-3 disaccharide units on both skeletal (type II) and corneal (type I) keratan sulfate chains either in isolated or proteoglycan-bound form. The epitope is resistant to proteolytic or alkaline cleavages and partially sensitive to keratanase or other endo-B-galactosidase treatment. |
| Isotype | IgG2b |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene encodes a member of the small leucine-rich proteoglycan (SLRP) family that includes decorin, biglycan, fibromodulin, keratocan, epiphycan, and osteoglycin. In these bifunctional molecules, the protein moiety binds collagen fibrils and the highly charged hydrophilic glycosaminoglycans regulate interfibrillar spacings. Lumican is the major keratan sulfate proteoglycan of the cornea but is also distributed in interstitial collagenous matrices throughout the body. Lumican may regulate collagen fibril organization and circumferential growth, corneal transparency, and epithelial cell migration and tissue repair. |
| Gene Symbol |
|
| UniProt Number |
|
| UniProt Summary | SIZE: 338 amino acids; 38429 Da SUBUNIT: Binds to laminin (By similarity). SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix (By similarity). TISSUE SPECIFICITY: Cornea and other tissues. DEVELOPMENTAL STAGE: Present in the extracellular matrix of human articular cartilage at all ages, although its abundance is far greater in the adult. In the adult cartilage lumican exists predominantly in a glycoprotein form lacking keratan sulfate, whereas the juvenile form of the molecule is a proteoglycan. SIMILARITY: SwissProt: P51884 ## Belongs to the small leucine-rich proteoglycan (SLRP) family. Class II subfamily. & Contains 12 LRR (leucine-rich) repeats. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at -20°C in convenient aliquots for up to 12 months. Avoid repeated freeze/thaw cycles. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |