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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | FC, IP, FUNC, IHC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB2250 |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Anti-Integrin β1 Antibody, clone 21C8 |
| Alternate Names |
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| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified immunoglobulin from Protein A Sepharose™ chromatography. Liquid in 0.02M PB pH 7.6, 0.25M NaCl containing 0.1% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Integrin β1 Antibody, clone 21C8 is an antibody against Integrin β1 for use in FC, IP, FUNC, IH. |
| Key Applications |
|
| Application Notes | Immunoprecipitation Immunohistochemistry on frozen sections Flow cytometry Stimulates adhesion of cells to extracellular matrix proteins (Wilkins et Al., 1996) Does not bind reduced beta1 integrin on Western blot Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | Jurkat T-leukemic cell line |
| Clone | 21C8 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | Reacts with the human integrin beta1 subunit. Specificity verified by preclearing of beta1in immunoprecipitation, flow cytometry on transfectant cells displaying human beta1 integrin, and reactivity with purified beta1. |
| Isotype | IgG1 |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P55268 # Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. SIZE: 1798 amino acids; 195981 Da SUBUNIT: Laminin is a complex glycoprotein, consisting of three different polypeptide chains (alpha, beta, gamma), which are bound to each other by disulfide bonds into a cross-shaped molecule comprising one long and three short arms with globules at each end. Beta-2 is a subunit of laminin-3 (S-laminin), laminin-4 (S- merosin), and laminin-7 (KS-laminin). SUBCELLULAR LOCATION: Secreted, extracellular space. Note=S- laminin is concentrated in the synaptic cleft of the neuromuscular junction. DOMAIN: SwissProt: P55268 The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure. & Domains VI and IV are globular. DISEASE: SwissProt: P55268 # Defects in LAMB2 are the cause of Pierson syndrome [MIM:609049]; also known as microcoria-congenital nephrotic syndrome. Pierson syndrome is characterized by nephrotic syndrome with neonatal onset, diffuse mesangial sclerosis and eye abnormalities with microcoria as the leading clinical feature. Death usually occurs within the first weeks of life. Disease severity depends on the mutation type: nontruncating LAMB2 mutations may display variable phenotypes ranging from a milder variant of Pierson syndrome to isolated congenital nephrotic syndrome. & Defects in LAMB2 are a cause of congenital nephrotic syndrome [MIM:609049]. Congenital nephrotic syndrome constitutes a heterogeneous group of conditions having in common the disruption of normal glomerular permselectivity. Congenital nephrotic syndrome due to LAMB2 mutations may be associated with ocular abnormalities. SIMILARITY: Contains 13 laminin EGF-like domains. & Contains 1 laminin IV type B domain. & Contains 1 laminin N-terminal domain. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at 2-8°C in undiluted aliquots for up to 6 months. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |